Long interspersed nuclear element (LINE)-1 methylation level as a molecular marker of early gastric cancer
| Autor: | Woo Chul Chung, Eun Jung Kim, Yeon-Ji Kim, Dae Bum Kim, Ji Min Lee, Yun Kyung Lee, Ji Han Jung |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty Biology Lesion 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Stomach Neoplasms Molecular marker Republic of Korea medicine Humans Aged Hepatology Gastroenterology Methylation DNA Methylation Middle Aged digestive system diseases Early Gastric Cancer Long interspersed nuclear element Core Binding Factor Alpha 3 Subunit Long Interspersed Nucleotide Elements 030104 developmental biology ROC Curve chemistry Gastric Mucosa Close relationship 030220 oncology & carcinogenesis Immunohistochemistry Line 1 methylation Female medicine.symptom |
| Zdroj: | Digestive and Liver Disease. 48:1093-1097 |
| ISSN: | 1590-8658 |
| DOI: | 10.1016/j.dld.2016.06.002 |
| Popis: | This study was performed to examine the state of long interspersed nuclear element (LINE)-1 methylation level in gastric epithelial dysplasias (GEDs) and evaluate as a molecular marker for gastric carcinogenesis when it was compared with RUNX3 expression.We examined 89 patients with GEDs subcategorized by the Vienna classification - 41 category 3 (low grade) and 48 category 4 (high grade/intramucosal carcinoma) lesion. All tissue samples were evaluated for RUNX3 immunohistochemical staining and the level of LINE-1 methylation.The rate of negative expression of RUNX3 in category 4 lesion was significant higher than category 3 (P0.01). LINE-1 methylation level was statistically different between category 3 and category 4 lesion (P0.01). Between positive and negative expression of RUNX3 in GEDs, there was a significant difference of LINE-1 methylation level (P0.01). The area under the ROC curve (AUC) of LINE-1 methylation level for diagnosis of category 4 lesion was 0.88 (95% CI, 0.76-1.00).LINE-1 methylation level was well correlated with the Vienna classification of GED and it had a close relationship with the negative expression of RUNX3 in category 4 lesion. LINE-1 methylation level could be a good candidate for a molecular marker of early gastric cancer. |
| Databáze: | OpenAIRE |
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