Novel cytological model for the identification of early oral cancer diagnostic markers: The carcinoma sequence model
| Autor: | Tatsuya Abé, Masami Kawaharada, Takeyasu Maeda, Taiichi Kitano, Manabu Yamazaki, Nyein Nyein Chan, Jun-ichi Tanuma, Tadaharu Kobayashi, Satoshi Maruyama |
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| Rok vydání: | 2022 |
| Předmět: |
p53
Pathology medicine.medical_specialty Cancer Research Sequence model immunocytochemistry 4-nitroquinoline 1-oxide Cytology medicine Carcinoma biochemistry liquid-based cytology business.industry Cancer Diagnostic marker Articles oral cancer medicine.disease oral squamous cell carcinoma stomatognathic diseases c-Myc Oncology Liquid-based cytology cytology Identification (biology) bromodomain protein 4 business |
| Zdroj: | Oncology Letters |
| ISSN: | 1792-1082 1792-1074 |
| DOI: | 10.3892/ol.2022.13196 |
| Popis: | Most oral squamous cell carcinomas (OSCCs) arise from a premalignant lesion, oral epithelial dysplasia; however, useful markers for the early detection of OSCC are lacking. The present study aimed to establish a novel experimental model to observe changes in the sequential expression patterns of mRNAs and proteins in a rat model of tongue cancer using liquid-based cytology techniques. Cytology specimens were collected at 2, 5, 8, 11, 14, 17 and 21 weeks from rats treated with 4-nitroquinoline 1-oxide to induce tongue cancer. The expression of candidate biomarkers was examined by performing immunocytochemistry and reverse transcription-quantitative PCR. The percentage of positively stained nuclei was calculated as the labeling index (LI). All rats developed OSCC of the tongue at 21 weeks. The mRNA expression levels of bromodomain protein 4 (Brd4), c-Myc and Tp53 were upregulated during the progression from negative for intraepithelial lesion or malignancy to squamous cell carcinoma (SCC). Brd4- and c-Myc-LI increased in low-grade squamous intraepithelial lesion, high-grade squamous intraepithelial lesion and SCC specimens. p53-LI was significantly increased in SCC specimens. This novel experimental model allowed the observation of sequential morphological changes and the expression patterns of mRNAs and proteins during carcinogenesis. Combining immunocytochemistry with cytology-based diagnoses may potentially improve the diagnostic accuracy of OSCC. |
| Databáze: | OpenAIRE |
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