The amyloid‐ β (A β ) peptide pattern in cerebrospinal fluid in Alzheimer's disease: evidence of a novel carboxyterminally elongated A β peptide
| Autor: | Eckart Rüther, Markus Otto, Juan Manuel Maler, Manuela Neumann, Johannes Kornhuber, Markus R. Meyer, Hermann Esselmann, Piotr Lewczuk, Volker Wollscheid, Jens Wiltfang |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Amyloid β Amyloid beta Peptide Mass spectrometry Mass Spectrometry Analytical Chemistry 03 medical and health sciences 0302 clinical medicine Cerebrospinal fluid Alzheimer Disease mental disorders Humans Spectroscopy Aged 030304 developmental biology Brain Chemistry chemistry.chemical_classification 0303 health sciences Amyloid beta-Peptides Postmortem brain biology Organic Chemistry Aβ peptide Control subjects Molecular biology 3. Good health Molecular Weight chemistry biology.protein Dementia Electrophoresis Polyacrylamide Gel Female 030217 neurology & neurosurgery |
| Zdroj: | Rapid Communications in Mass Spectrometry. 17:1291-1296 |
| ISSN: | 1097-0231 0951-4198 |
| DOI: | 10.1002/rcm.1048 |
| Popis: | The patterns of amyloid beta (Abeta) peptides in human cerebrospinal fluid (CSF) and brain homogenates were studied by surface-enhanced laser desorption/ionization (SELDI) time-of-flight (TOF) mass spectrometry, and the results were compared with those obtained by Abeta-SDS-PAGE/immunoblot. Apart from the peptides known in the literature to occur in the CSF, we postulate the existence of a novel, previously not described peptide, either Abeta1-45 or Abeta2-46. This peptide was observed exclusively in a pool of samples originating from patients with AD, i.e. CSF and postmortem brain homogenates, but not in either the pooled CSF samples nor the pooled brain homogenates of the non-demented controls. Similarly to our previous results, Abeta1-42 was decreased in the CSF in AD. Expectedly, brain homogenates of the control subjects did not show the presence of Abeta peptides. Compared with Abeta-SDS-PAGE/immunoblot, SELDI-TOF enabled more precise analysis of Abeta peptides in the human material. We conclude that SELDI-TOF offers a promising tool for dementia expression pattern profiling using a minute amount of a biological sample. |
| Databáze: | OpenAIRE |
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