A mixture of milk and vegetable lipids in infant formula changes gut digestion, mucosal immunity and microbiota composition in neonatal piglets

Autor: Karima Bouzerzour, Claire Bourlieu, Pascale Le Ruyet, Cécile Bonhomme, Didier Dupont, Cindy Le Bourgot, Isabelle Le Huërou-Luron, Julien Jardin, Cécile Perrier, Stéphanie Ferret-Bernard, Laurence Le Normand, Olivia Ménard, Isabelle Cuinet, Thomas Carton
Přispěvatelé: Nutrition, Métabolismes et Cancer (NuMeCan), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM), Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Biofortis, R&D, Grp Lactalis, Lactalis Nutrition Santé, Lactalis company, Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Sus scrofa
Medicine (miscellaneous)
Lactoglobulins
Plant Proteins
Dietary
Feces
fluids and secretions
Casein
Food science
Infant Nutritional Physiological Phenomena
2. Zero hunger
Nutrition and Dietetics
biology
Chemistry
Milk fat
Microbiota
Caseins
Gastrointestinal Contents
3. Good health
medicine.anatomical_structure
Milk
Infant formula
Cytokines
Animal Nutritional Physiological Phenomena
Digestion
Protein digestion
Firmicutes
Ileum
Intestinal immune system
Breast milk
Intestinal permeability
Immunomodulation
03 medical and health sciences
medicine
Animals
Humans
Plant Oils
Immunity
Mucosal
Glycoproteins
030109 nutrition & dietetics
Infant
Newborn
Models
Immunological
Lipid Droplets
biology.organism_classification
Gastrointestinal Microbiome
Animals
Newborn
Cytokine secretion
Lymph Nodes
Glycolipids
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Zdroj: European Journal of Nutrition
European Journal of Nutrition, Springer Verlag, 2018, 57 (2), pp.463-476. ⟨10.1007/s00394-016-1329-3⟩
ResearcherID
European Journal of Nutrition, 2018, 57 (2), pp.463-476. ⟨10.1007/s00394-016-1329-3⟩
ISSN: 1436-6207
1436-6215
Popis: The online version of this article (doi:10.1007/s00394-016-1329-3) contains supplementary material, which is available to authorized users.; International audience; Purpose : Although composition of infant formula has been significantly improved during the last decade, major differences with the composition and structure of breast milk still remain and might affect nutrient digestion and gut biology. We hypothesized that the incorporation of dairy fat in infant formulas could modify their physiological impacts by making their composition closer to that of human milk. The effect of milk fat and milk fat globule membrane (MFGM) fragments in infant formulas on gut digestion, mucosal immunity and microbiota composition was evaluated. Methods : Three formulas containing either (1) vegetable lipids stabilized only by proteins (V-P), (2) vegetable lipids stabilized by a mixture of proteins and MFGM fragments (V-M) and (3) a mixture of milk and vegetable lipids stabilized by a mixture of proteins and MFGM fragments (M-M) were automatically distributed to 42 newborn piglets until slaughter at postnatal day (PND) 7 or 28, and compared to a fourth group of sow’s suckling piglets (SM) used as a breast-fed reference. Results : At both PND, casein and β-lactoglobulin digestion was reduced in M-M proximal jejunum and ileum contents compared to V-P and V-M ones leading to more numerous β-Cn peptides in M-M contents. The IFNγ cytokine secretion of ConA-stimulated MLN cells from M-M piglets tended to be higher than in V-P ones at PND 7 and PND 28 and was closer to that of SM piglets. No dietary treatment effect was observed on IL-10 MLN cell secretion. Changes in faecal microbiota in M-M piglets resulted in an increase in Proteobacteria and Bacteroidetes and a decrease in Firmicutes phyla compared to V-P ones. M-M piglets showed higher abundances of Parabacteroides, Escherichia/Shigella and Klebsiella genus. Conclusions : The incorporation of both milk fat and MFGM fragments in infant formula modifies protein digestion, the dynamic of the immune system maturation and the faecal microbiota composition.
Databáze: OpenAIRE
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