Molecularly imprinted polymer cartridges coupled to high performance liquid chromatography (HPLC-UV) for simple and rapid analysis of fenthion in olive oil
| Autor: | Elena V. Piletska, Thierry Noguer, Idriss Bakas, Georges Istamboulie, Sergey A. Piletsky, Najwa Ben Oujji, Régis Rouillon, Elhabib Ait-Addi, Ihya Ait-Ichou |
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| Rok vydání: | 2014 |
| Předmět: |
Models
Molecular Detection limit Insecticides Acetonitriles Chromatography Fenthion Polymers Ethylene glycol dimethacrylate Extraction (chemistry) Molecularly imprinted polymer Food Contamination High-performance liquid chromatography Chemistry Techniques Analytical Analytical Chemistry Molecular Imprinting chemistry.chemical_compound chemistry Limit of Detection Acrylamide Plant Oils Solid phase extraction Olive Oil Chromatography High Pressure Liquid |
| Zdroj: | Talanta. 125:313-318 |
| ISSN: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2014.03.020 |
| Popis: | A combination of molecular modelling and a screening of the library of non-imprinted polymers (NIPs) was used to identify acrylamide as a functional monomer with high affinity towards fenthion, organophosphate insecticide, which is frequently used in the treatment of olives. A good correlation was found between the screening tests and modelling of monomer-template interactions performed using a computational approach. Acrylamide-based molecularly imprinted polymer (MIP) and non-imprinted polymer (NIP) were thermally synthesised in dimethyl formamide (porogen) using ethylene glycol dimethacrylate as a cross-linker and 1,1-azo-bis (isobutyronitrile) as an initiator. The chemical and physical properties of the prepared polymers were characterised. The binding of fenthion by the polymers was studied using solvents with different polarities. The developed MIP showed a high selectivity towards fenthion, compared to other organophosphates (dimethoate, methidathion malalthion), and allowed extraction of fenthion from olive oil samples with a recovery rate of about 96%. The extraction of fenthion using MIPs was much more effective than traditional C18 reverse-phase solid phase extraction and allowed to achieve a low detection limit (LOD) (5 µg L(-1)). |
| Databáze: | OpenAIRE |
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