Expression Profile of LncRNAs in Childhood Acute Lymphoblastic Leukemia: A Pilot Study
| Autor: | Saeid Ghorbian, Leyla Roshangar, Zohreh Mousavi, Azim Rezamand, Behboud Jafari |
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| Rok vydání: | 2020 |
| Předmět: |
immune-phenotype
Oncology medicine.medical_specialty long non-coding rna Pharmaceutical Science acute lymphoblastic leukemia medicine.disease_cause 030226 pharmacology & pharmacy 01 natural sciences 03 medical and health sciences Pharmacy and materia medica 0302 clinical medicine oncogenesis Transcription (biology) Internal medicine Complementary DNA medicine In patient General Pharmacology Toxicology and Pharmaceutics Gene Childhood Acute Lymphoblastic Leukemia business.industry 010401 analytical chemistry Lncrna expression eye diseases Long non-coding RNA 0104 chemical sciences RS1-441 business Carcinogenesis |
| Zdroj: | Pharmaceutical Sciences, Vol 27, Iss 3, Pp 385-392 (2021) |
| ISSN: | 2383-2886 1735-403X |
| DOI: | 10.34172/ps.2020.84 |
| Popis: | Background: Childhood acute lymphoblastic leukemia (ALL) explains 26% of pediatricmalignancies and is one of the leading causes of disease-related deaths in children. A novelmolecular class of non-coding genes, long non-coding RNAs (lncRNAs) having over 200nucleotides, have been defined as regulators of different cellular processes including pluripotency,oncogenesis, and transcription. It has been demonstrated that lncRNA transcription profilescan distinguish pre B-cell subtype of ALL accurately and act as early diagnostic and prognosticbiomarkers. Hence, the aim of this pilot study was the prior evaluation of expression profileof several lncRNA candidates including RP11-68I18.10, RP11-624C23.1, RP11-446E9, RP11-137H2.4, and RP11-203E8 in patients with ALL. Methods: In this study, 80 blood samples were obtained from patients, definitely diagnosed bypathologists with ALL, and from healthy subjects. Total RNA was extracted from blood samples,and cDNA was synthesized. Real-time PCR was applied to determine the expression of lncRNAs.A P-value of 0.010 was considered statistically significant. Results: Our findings revealed that the expression levels of lncRNAs RP11-624C23.1, RP11-446E9, RP11-137H2.4, RP11-68I18.10, and RP11-203E8 were significantly decreased in ALLsamples compared to those of healthy samples (P |
| Databáze: | OpenAIRE |
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