Usefulness of the polymerase chain reaction dot-blot assay, used with Ziehl-Neelsen staining, for the rapid and convenient diagnosis of pulmonary tuberculosis in human immunodeficiency virus-seropositive and -seronegative individuals
| Autor: | Rosa Dea Sperhacke, Luciene Cardoso Scherer, Afrânio Lineu Kritski, Maria Lucia Rosa Rossetti, Antonio Ruffino-Netto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
in-house polymerase chain reaction Tuberculosis Prevalence Dot blot Gastroenterology Article in house PCR HIV Tuberculosis law.invention 03 medical and health sciences symbols.namesake 0302 clinical medicine tuberculosis law Internal medicine human immunodeficiency virus Medicine 030212 general & internal medicine Fisher's exact test Polymerase chain reaction 0303 health sciences 030306 microbiology business.industry lcsh:Other systems of medicine medicine.disease lcsh:RZ201-999 3. Good health Staining Infectious Diseases Immunology symbols Sputum Ziehl–Neelsen stain medicine.symptom business |
| Zdroj: | Infectious Disease Reports, Vol 3, Iss 1, Pp e3-e3 (2011) Infectious Disease Reports; Volume 3; Issue 1; Pages: e3 Infectious Disease Reports |
| ISSN: | 2036-7449 2036-7430 |
| Popis: | There are scarce data regarding the value of molecular tests, when used in parallel with classical tools, for the diagnosis of tuberculosis (TB) under field conditions, especially in regions with a high burden of TB-human immunodeficiency virus (HIV) co-infection. We evaluated the usefulness of the polymerase chain reaction dot-blot assay (PCR) used in parallel with Ziehl-Neelsen staining (ZN) for pulmonary tuberculosis (PTB) diagnosis, in a TB-HIV reference hospital. All sputum samples from 277 patients were tested by ZN, culture, and PCR. Performances were assessed individually, in parallel, for HIV status, history of anti-TB treatment, and in different simulated TB prevalence rates. Overall, the PTB prevalence was 46% (128/277); in HIV-seropositive (HIV+) individuals, PTB prevalence was 54% (40/74); the ZN technique had a lower sensitivity (SE) in the HIV+ group than in the HIV-seronegative (HIV–) group (43% vs. 68%; Fisher test, P |
| Databáze: | OpenAIRE |
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