Biochemical Effects of Routine Gonadectomy on Blood of Domestic Ferrets (Mustela putorius furo)
| Autor: | Michelle C. Whitehead, Mary Doerr, Tara M. Harrison, Michael K. Stoskopf, Olivia A. Petritz |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty Metabolite chemistry.chemical_compound Alloisoleucine Internal medicine Animals Medicine Castration Experimental Use Whole blood chemistry.chemical_classification biology business.industry Ferrets biology.organism_classification Amino acid Endocrinology chemistry Mustela putorius biology.protein Female Animal Science and Zoology Creatine kinase Leucine Isoleucine business |
| Zdroj: | J Am Assoc Lab Anim Sci |
| ISSN: | 1559-6109 |
| DOI: | 10.30802/aalas-jaalas-19-000173 |
| Popis: | We studied domestic ferrets (Mustela putorius furo) to evaluate the physiologic effects of routine surgery. Standard plasma biochemistry panels and 1H-NMR spectroscopy of heparinized whole blood were performed on samples taken 24 h prior to and immediately after surgery from female and male ferrets undergoing routine gonadectomy. Increases in plasma glucose, phosphorus, potassium, and creatine kinase concentrations associated with the duration of surgery were identified on plasma biochemistry panels. Whole-blood NMR spectra allowed us to identify 42 metabolites and one drug residue. Variations between pre- and postoperative metabolite concentrations were most pronounced for female ferrets, which underwent more prolonged surgery than males. Affected metabolites included organic acids and osmolytes (betaine, methylmalonate, D-lactate), fatty acids and lipids (2-hydroxy-3-methylbutyric acid), and amino acid groups (acetylglycine, alloisoleucine, leucine, and isoleucine). These findings indicate that 1H-NMR spectroscopy of whole blood provides insight into metabolic perturbations in domestic ferrets undergoing surgery that are not detected in routine clinical chemistry panels. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|