Relationship between the gingival sulcus depth and interleukin-1 isoform concentrations within the adjacent gingival tissue

Autor: Jennifer L. Bain, Francis G. Serio, Roger B. Johnson, S. R. Lester
Rok vydání: 2009
Předmět:
Zdroj: Journal of Periodontal Research. 44:323-329
ISSN: 1600-0765
0022-3484
DOI: 10.1111/j.1600-0765.2008.01136.x
Popis: Background and Objective: While there is substantial information concerning the concentrations of interleukin-1 isoforms within gingival crevicular fluid, there is little information concerning their concentrations within either normal or diseased gingival tissues. Therefore, the aim of this study was to evaluate the relationship between the concentrations of gingival interleukin-1 isoforms and the adjacent sulcular depth. Material and Methods: Interdental gingival papillae were excised and grouped based on adjacent pocket depth and the presence of bleeding on probing. Gingiva adjacent to a sulcus of ≤ 3 mm without bleeding on probing were classified as ‘normal’; gingiva adjacent to a 3-mm sulcus with bleeding on probing were classified as ‘diseased-slight’; gingiva adjacent to a 4–6-mm sulcus featuring bleeding on probing were classified as ‘diseased-moderate’; and gingiva adjacent to a sulcus of > 6 mm featuring bleeding on probing were classified as ‘diseased-severe’. Tissues were solublized and the concentrations of interleukin-1β, interleukin-1α, interleukin-1 receptor antagonist and interleukin-6 were assessed by enzyme-linked immunosorbent assay. Data were compared by factorial analysis of variance, the post-hoc Tukey test and the Pearson’s correlation test. Results: Gingival concentrations of interleukin-6, interleukin-1 receptor antagonist, interleukin-1α- and interleukin-1β were significantly greater at diseased-severe sites than at normal, diseased-slight, or diseased-moderate sites (p
Databáze: OpenAIRE