Identification and functional analysis of the cathepsin D gene promoter of Bombyx mori
| Autor: | Fengming Zou, Guo-Zheng Zhang, Byung-Rae Jin, Yan-Wei Liu, Fang Yin, Jun-Qiang Jia, Jie Yu, Xiang-Yun Cai, Feng-Yao Wu, Zhong-Zheng Gui, Hai-Yan Yu, Zi-Xu Ren, Xijie Guo |
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| Rok vydání: | 2014 |
| Předmět: |
biology
viruses fungi Gene Expression Regulation Developmental Cathepsin D Promoter General Medicine Bombyx biology.organism_classification Molecular biology law.invention Autographa californica Organ Specificity Bombyx mori Transcription (biology) law Larva Genetics Recombinant DNA Animals Ectopic expression Promoter Regions Genetic Molecular Biology Gene |
| Zdroj: | Molecular Biology Reports. 41:1623-1630 |
| ISSN: | 1573-4978 0301-4851 |
| DOI: | 10.1007/s11033-013-3009-1 |
| Popis: | The gene encoding cathepsin D of silkworm, Bombyx mori (BmCatD) is specifically expressed in the larval fat body and pupal gut, and plays an important role in the programmed cell death during metamorphosis. To identify element involved in this transcription-dependent spatial restriction, truncation and deletion of the 5' terminal from the BmCatD promoter were conducted in vivo. The recombinant AcMNPV vector (Autographa californica multiple nucleopolyhedrovirus) with a dual-luciferase quantitative assay system was used as the transfer. A 289 bp DNA sequence (-1,214 to -925) upstream of the transcriptional start site is found to be responsible for promoting tissue-specific transcription. Further analysis of a series of deletion within the 289 bp region of overlapping deletion showed that a 33 bp region (-1,071 to -1,038) sequence suppresses the ectopic expression of the BmCatD promoter. These results suggest that this 33 bp region could function as a promoter element in the tissue-specificity expression. |
| Databáze: | OpenAIRE |
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