LncRNA BASP1-AS1 interacts with YBX1 to regulate Notch transcription and drives the malignancy of melanoma

Autor: YaLi Gao, Xueli Niu, Bing Song, MingSui Tang, Xiu-Hao Guan, JingYi Li, Yaling Li
Rok vydání: 2021
Předmět:
Male
Cancer Research
Skin Neoplasms
Transcription
Genetic
Carcinogenesis
medicine.disease_cause
YBX1
Mice
Random Allocation
Transcription (biology)
Cell Movement
NOTCH3
Copy-number variation
Receptor
Notch3
Mutation
Mice
Inbred BALB C
Melanoma
GTPase-Activating Proteins
General Medicine
Prognosis
Long non-coding RNA
Neoplasm Proteins
Up-Regulation
BASP1‐AS1
Oncology
Proto-Oncogene Proteins c-bcl-2
Neoplastic Stem Cells
RNA
Long Noncoding
Original Article
signature
Notch signaling pathway
Nerve Tissue Proteins
Biology
Cell Line
Tumor
medicine
melanoma
Animals
Humans
Neoplasm Invasiveness
Gene Silencing
Gene
Cell Proliferation
Membrane Proteins
Original Articles
medicine.disease
Repressor Proteins
Cancer research
Murine pneumonia virus
Ectopic expression
Y-Box-Binding Protein 1
Neoplasm Transplantation
Zdroj: Cancer Science
ISSN: 1349-7006
1347-9032
Popis: Melanoma is a fatal skin malignant tumor with a poor prognosis. We found that long noncoding RNA BASP1 ‐AS1 is essential for the development and prognosis of melanoma. The methylation, RNA sequencing, copy number variation, mutation data, and sample follow‐up information of melanoma from The Cancer Genome Atlas (TCGA) were analyzed using weighted gene co‐expression network analysis and 366 samples common to the three omics were selected for multigroup clustering analysis. A four‐gene prognostic model (BASP1‐AS1, LOC100506098, ARHGAP27P1, and LINC01532) was constructed in the TCGA cohort and validated using the GSE65904 series. The expression of BASP1‐AS1 was upregulated in melanoma tissues and various melanoma cell lines. Functionally, the ectopic expression of BASP1‐AS1 promoted cell proliferation, migration, and invasion in both A375 and SK‐MEL‐2 cells. Mechanically, BASP1‐AS1 interacted with YBX1 and recruited it to the promoter of NOTCH3, initiating its transcription process. The activation of the Notch signaling then resulted in the transcription of multiple oncogenes, including c‐MYC, PCNA, and CDK4, which contributed to melanoma progression. Thus, BASP1‐AS1 could act as a potential biomarker for cutaneous malignant melanoma.
We identified a new type of long noncoding RNA (LncRNA) BASP1 ‐AS1, which can promote melanoma development both in vivo and in vitro. Detailed studies on the molecular mechanism showed that BASP1‐AS1 promoted the proliferation, invasion, and migration of melanoma cells by regulating YBX1. In addition, LncRNA BASP1‐AS1 is a poor prognostic indicator and a potential therapeutic target for melanoma.
Databáze: OpenAIRE
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