Protein Expression in Tonsillar and Base of Tongue Cancer and in Relation to Human Papillomavirus (HPV) and Clinical Outcome
| Autor: | Eva Munck-Wikland, Linnea Haeggblom, Andrey Alexeyenko, Cinzia Bersani, Tina Dalianis, Aeneas Kolev, Susanne Becker, Anders Näsman, Torbjörn Ramqvist, Bo Franzén |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Proteomics Vascular Endothelial Growth Factor A Chemokine Angiogenesis oropharyngeal cancer Tonsillar Neoplasms clinical outcome base of tongue cancer lcsh:Chemistry 0302 clinical medicine Hypoxia human papillomavirus lcsh:QH301-705.5 Papillomaviridae Spectroscopy Aged 80 and over Immunity Cellular biology Neovascularization Pathologic tonsillar cancer proximity extension assay protein expression virus diseases General Medicine Middle Aged female genital diseases and pregnancy complications Computer Science Applications Tongue Neoplasms Vascular endothelial growth factor A 030220 oncology & carcinogenesis Carcinoma Squamous Cell Immunohistochemistry Base of tongue cancer Female medicine.symptom Adult Catalysis Article Inorganic Chemistry 03 medical and health sciences Immune system medicine Humans Physical and Theoretical Chemistry Molecular Biology Aged business.industry Organic Chemistry Papillomavirus Infections Cancer Hypoxia (medical) medicine.disease Survival Analysis 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Protein Biosynthesis Mutation biology.protein Cancer research business |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 19, Iss 4, p 978 (2018) International Journal of Molecular Sciences; Volume 19; Issue 4; Pages: 978 |
| ISSN: | 1422-0067 |
| Popis: | Human papillomavirus (HPV) is a major etiological factor for tonsillar and the base of tongue cancer (TSCC/BOTSCC). HPV-positive and HPV-negative TSCC/BOTSCC present major differences in mutations, mRNA expression and clinical outcome. Earlier protein studies on TSCC/BOTSCC have mainly analyzed individual proteins. Here, the aim was to compare a larger set of cancer and immune related proteins in HPV-positive and HPV-negative TSCC/BOTSCC in relation to normal tissue, presence of HPV, and clinical outcome. Fresh frozen tissue from 42 HPV-positive and 17 HPV-negative TSCC/BOTSCC, and corresponding normal samples, were analyzed for expression of 167 proteins using two Olink multiplex immunoassays. Major differences in protein expression between TSCC/BOTSCC and normal tissue were identified, especially in chemo- and cytokines. Moreover, 34 proteins, mainly immunoregulatory proteins and chemokines, were differently expressed in HPV-positive vs HPV-negative TSCC/BOTSCC. Several proteins were potentially related to clinical outcome for HPV-positive or HPV-negative tumors. For HPV-positive tumors, these were mostly related to angiogenesis and hypoxia. Correlation with clinical outcome of one of these, VEGFA, was validated by immunohistochemistry. Differences in immune related proteins between HPV-positive and HPV-negative TSCC/BOTSCC reflect the stronger activity of the immune defense in the former. Angiogenesis related proteins might serve as potential targets for therapy in HPV-positive TSCC/BOTSCC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|