Caracterização de enzimas digestivas do linguado brasileiro de cativeiro Paralichthys orbignyanus
| Autor: | R. C. A. Neri, Marcelo Borges Tesser, F. B. Candiotto, Ricardo Vieira Rodrigues, Luís André Sampaio, A. C. V. Freitas-Júnior, Ranilson de Souza Bezerra |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
medicine.medical_treatment Flounder digestive tract 01 natural sciences Aminopeptidase Aminopeptidases Pepsin lcsh:Botany lcsh:Zoology Enzyme Stability Amylase Food science lcsh:QL1-991 lcsh:Science lcsh:QH301-705.5 chemistry.chemical_classification biology Serine Endopeptidases Temperature 04 agricultural and veterinary sciences Hydrogen-Ion Concentration Trypsin lcsh:QK1-989 trypsin Liver General Agricultural and Biological Sciences trato digestório Brazil medicine.drug Fish Proteins medicine Animals Protease 010604 marine biology & hydrobiology protease biology.organism_classification Enzyme assay Fishery Gastrointestinal Tract Enzyme chemistry lcsh:Biology (General) 040102 fisheries biology.protein 0401 agriculture forestry and fisheries lcsh:Q proteases tripsina |
| Zdroj: | Brazilian Journal of Biology, Issue: ahead, Published: 17 AUG 2017 Brazilian Journal of Biology v.78 n.2 2018 Brazilian Journal of Biology Instituto Internacional de Ecologia (IIE) instacron:IIE Brazilian Journal of Biology, Iss 0 (2017) Brazilian Journal of Biology, Volume: 78, Issue: 2, Pages: 281-288, Published: 17 AUG 2017 |
| Popis: | Knowledge of specific enzyme activity, along with animal habits and digestive capacity is essential in formulating an appropriate diet for any species. In this study, we evaluated and characterized the activity of digestive enzymes present in the liver, intestine, and stomach of Paralichthys orbignyanus. The effects of pH and temperature on enzyme activity were also evaluated via the use of specific substrates. The use of specific substrates and inhibitors showed strong evidence of the presence of trypsin (BApNA= 0.51 ± 0.2 mU mg-1), chimotrypsin (SApNA= 2.62 ± 1.8 mU mg-1), and aminopeptidases (Leu-p-Nan =0.9709 ± 0.83 mU mg-1) in the intestine. Optimum pH for the activity of trypsin, chemotrypsin, leucino aminopeptidase, amilase, and pepsin were 9.5, 9.0, 8.0, 7.5, and 3.5, respectively, while optimum temperatures were 50, 50, 50, 40, and 45 °C, respectively. These results provide additional information regarding the biology of Brazilian flounder and can be used as a basis for further studies regarding fish feeding physiology. Resumo O conhecimento da atividade enzimática é essencial para formular uma correta dieta específica para espécie, além de estarem correlacionadas com o hábito da alimentação e capacidade digestive. Neste estudo determinamos e caracterizamos a atividade enzimática presente no intestino, estômago e fígado do linguado Paralichthys orbignyanus. Os efeitos da temperatura e pH sobre a atividade enzimática também foram avaliados utilizando substratos específicos. O uso de substratos e inibidores específicos mostrou uma forte evidência da presença da tripsina (BApNA = 0,51 ± 0,2 mU mg-1), quimotripsina (SAPNA = 2,62 ± 1,8 mU mg-1), e as aminopeptidases (Leu-p-Nan = 0,97 ± 0,83 mU mg-1) no intestino. O pH ótimo observado para a atividade de tripsina, quimotripsina, leucino aminopeptidase, amilase e pepsina foi 9,5, 9,0, 8,0, 7,5 e 3,5, respectivamente. A temperatura ótima observada foi 50, 50, 50, 40 e 45 °C, respectivamente. Estes resultados fornecem informações adicionais sobre a biologia do linguado brasileiro e pode ser usado como base para novos estudos sobre fisiologia alimentar. |
| Databáze: | OpenAIRE |
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