SPECTRUM OF ANTIBIOTIC RESISTANCE AND PREVALENCE OF OXA-CARBAPENEMASES AMONG ACINETOBACTER BAUMANNII STRAINS, ISOLATED FROM PATIENTS OF SURGICAL AND REANIMATION DEPARTMENTS IN MOSCOW
| Autor: | Mayansky Na, Bocharova Ya, Kryzhanovskaya Oa, A.V. Lazareva, Chebotar |
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| Předmět: |
0301 basic medicine
carbapenemase genes medicine.drug_class 030106 microbiology Antibiotics Medicine (miscellaneous) Microbiology antibiotics law.invention resistance 03 medical and health sciences Antibiotic resistance law polycyclic compounds medicine Polymerase chain reaction biology business.industry General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification QR1-502 Acinetobacter baumannii Ciprofloxacin Colistin bacteria Gentamicin Netilmicin acinetobacter baumannii business medicine.drug |
| Zdroj: | Scopus-Elsevier ResearcherID Журнал микробиологии, эпидемиологии и иммунобиологии, Vol 0, Iss 1, Pp 40-45 (2016) |
| Popis: | Aim. Characterize spectrum of antibiotics resistance of Acinetobacter baumannii strains, isolated from patients of 8 surgical and reanimation departments of 3 medical institution of Moscow, and determine molecular-genetic mechanisms of stability of their carbapenem-resistant forms. Materials and methods. 95 strains of A. baumannii, isolated from patients of reanimation and surgical departments of Moscow in 2012 - 2014, were studied. Sensitivity of strains to antibiotics was tested phenotypically according to recommendations of EUCAST. The presence ofVIM, IMP, OXA-23, OXA-40, OXA-48, OXA-58 and NDM genes in the studied strains was determined by polymerase chain reaction in real time. Results. 86.3% of strains turned out to be non-sensitive to carbapenems, sensitive - 13.7%. 80.0% of strains were non-sensitive to gentamicin, 80.0% of strains - to netilmicin, 94.7% of strains - to ciprofloxacin, 2.1% - to colistin. 91.6% of isolates have shown non-sensitivity to members of 2 and more classes of antibiotics, 78.9% of strains - to members of 3 classes. 2 strains were panresistant, 4.2% (4/95) of the isolates were sensitive to all the classes of antibiotics. Metallo-P-lactamases were not detected. Genes of carbapenemases (OXA-23 and/or OXA-40) were detected in 85.3% (81/95) of strains, characterized phenotypically as non-sensitive to carbapenems. Conclusion. The results obtained shown an increase of resistance to carbapenems and multiple resistance in clinically significant strains of A. baumannii. Resistance to carbapenems is associated with OXA-23 and OXA-40 genes. The conclusions allow to justify perspectives of introduction of technologies of molecular-genetic testing of antibiotics resistance. |
| Databáze: | OpenAIRE |
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