Recombinant OmpA protein fragments mediate interleukin-17 regulation to prevent Escherichia coli meningitis
| Autor: | Chia Chih Chang, Hsueh Hsia Wu, Pei Hsuan Lin, Wen Shyang Hsieh, Yi Yuan Yang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Microbiology (medical) Chemokine Meningitis Escherichia coli medicine.medical_treatment Nitric Oxide Synthase Type II medicine.disease_cause Neonatal meningitis Microbiology law.invention 03 medical and health sciences 0302 clinical medicine law Immunology and Microbiology(all) Cell Line Tumor Escherichia coli medicine Animals Humans Immunology and Allergy Escherichia coli infection General Immunology and Microbiology biology Interleukin-17 Infant Newborn General Medicine medicine.disease Virology outer membrane protein A Peptide Fragments Rats NOS-2 Mice Inbred C57BL Nitric oxide synthase IL-17 030104 developmental biology Infectious Diseases Cytokine Cyclooxygenase 2 biology.protein Recombinant DNA Interleukin 17 bacterial meningitis 030217 neurology & neurosurgery Bacterial Outer Membrane Proteins |
| Zdroj: | Journal of Microbiology, Immunology and Infection. 49:843-850 |
| ISSN: | 1684-1182 |
| DOI: | 10.1016/j.jmii.2015.05.019 |
| Popis: | Background Neonates are at a higher risk for bacterial meningitis than children of other age groups. Although the mortality rates have decreased over the past few decades, neonatal meningitis is still a severe disease with high morbidity. For bacterial meningitis, antibiotic therapy is the primary choice for management. However, neurologic complications often cannot be averted; ∼40% of survivors exhibit neurological sequelae. Escherichia coli infection is the common cause of neonatal meningitis. Previously, we have demonstrated that the recombinant loop 1–3, loop 2–3, and loop 2–4 fragments of OmpA protein can protect mice from death after intracerebral E. coli infection. In this study, the protective effects of the recombinant OmpA protein fragments in E. coli intracerebral infections were investigated. Methods The effects of E. coli intracerebral infection on cytokine and chemokine expression were determined. We also used various recombinant fragments of the OmpA protein to investigate the effects of these recombinant OmpA protein fragments on cytokine and chemokine expression. Results In this study, we demonstrated that the expression of interleukin-17 and other cytokines, chemokines, inducible nitric oxide synthase, and cyclooxygenase-2 are involved in the inflammatory processes of intracerebral E. coli infection. We also demonstrated that specific recombinant OmpA protein fragments (L1–3, L2–3, L2–4, and L3) can regulate cytokine, chemokine, nitric oxide synthase, and cyclooxygenase-2 expression and, subsequently, protect mice from death caused by intracerebral infection of E. coli . Conclusion This finding indicates the potential for developing a new therapeutic approach to improve the prognosis of bacterial meningitis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|