The Domestic Environment and the Lung Mycobiome
| Autor: | David Orts, Jorge Adsuar, José Franco, Beatriz Gálvez, María Francisca Colom, Enrique Gómez-Imbernón, Beatriz Amat, Cleofé Fernández, Violeta Esteban, Esther Rubio-Portillo, Consuelo Ferrer, Eleuterio Llorca, Carlos Pérez-Martín, Josefa Antón, Elena Revelles, Pedro Piqueras |
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| Přispěvatelé: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología, Ecología Microbiana Molecular |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Mycobiota Bronco-alveolar lavage 030106 microbiology Biology Microbiología Microbiology Article 03 medical and health sciences mycobiome lower respiratory tract Domestic environment Virology medicine Internal transcribed spacer lcsh:QH301-705.5 Lung house dust Lower respiratory tract Fungi bronco-alveolar lavage House dust DNA extraction 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) Potato dextrose agar fungi mycobiota Mycobiome |
| Zdroj: | RUA. Repositorio Institucional de la Universidad de Alicante Universidad de Alicante (UA) Microorganisms, Vol 8, Iss 1717, p 1717 (2020) Microorganisms Volume 8 Issue 11 |
| Popis: | This study analyzes the relationship between the mycobiome of the Lower Respiratory Tract (LRT) and the fungi in the domestic environment. Samples studied consisted of Broncho-Alveolar Lavage (BAL) from 45 patients who underwent bronchoscopy for different diagnostic purposes, and dust and air from the houses (ENV) of 20 of them (44.4%). Additionally, five bronchoscopes (BS) were also analyzed and negative controls were included for every procedure. All samples were processed for DNA extraction and cultures, which were performed in Sabouraud Dextrose and Potato Dextrose Agar. The fungal Internal Transcribed Spacer (ITS2) was sequenced by the Solexa/Illumina system and sequences were analyzed by QIIME 1.8.0 and compared with the UNITE Database for identification. The similarity between the two fungal communities (BAL and ENV) for a specific patient was assessed via the percentage of coincidence in the detection of specific operational taxonomic units (OTUs), and about 75% of co-occurrence was detected between the mycobiome of the LRT and the houses. Cultures confirmed the presence of the core mycobiome species. However, the low rate of isolation from BAL suggests that most of its mycobiome corresponds to non-culturable cells. This likely depends on the patient&rsquo s immune system activity and inflammatory status. |
| Databáze: | OpenAIRE |
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