| Autor: |
Michael J. Ellison, Lei Li, John J.M. Bergeron, David W. Speicher, Marina Hincapie, Sjoerd van der Post, Peter J. Sheffield, Catherine C. L. Wong, Thomas A. Beardslee, P. D. Semchuk, Sean C. Bendall, Daniel Cociorva, Wantao Ying, Caroline May, Seul Ki Jeong, Wei Jia, Philip C. Andrews, Steve A. Carr, Benoit Houle, Robert L. Moritz, Lennart Martens, Ana Lopez-Campistrous, Martin Eisenacher, Alexander W. Bell, Raju Kucherlapati, Ron Beavis, Xiaohong Qian, Fuchu He, Brian L. Hood, John R. Yates, Tommy Nilsson, Kieran Wynne, Radoslav Goldman, Vivian Nguyen, Katy Williams, Giuliano Elia, Rudolf Grimm, Salvatore Sechi, Christoph H. Borchers, Yanming An, Masanori Fujimoto, Catherine E. Au, Young Ki Paik, Mahbod Hajivandi, Peipei Ping, Yun Cai, Pavel Metalnikov, Christine A. Miller, Christian Stephan, Wenhong Zhu, Rushdy Ahmad, Marshall Pope, Jinglan Wang, Thomas P. Conrads, Eric W. Deutsch, Min-Seok Kwon, Gilles A. Lajoie, Michael J. Dunn, Guy G. Poirier, Juan Antonio Vizcaíno, William L. Bigbee, Lina Song, John R. Strahler, Sang Yun Cho, Phillip Gray, Craig A. Dorschel, William S. Hancock, Tony Pawson, Kenneth C. Parker, Kaye D. Speicher, Angela K. Walker, Paul F. Predki, Heather Patsiouras, Richard J. Simpson, Thomas Chappell, Kenneth E. Hung, Qing Zhang, Samir M. Hanash, Eugene A. Kapp, Yueju Wang, Katrin Marcus, Jason J. Struthers, Gavin Meredith, Junying Wei, Yangjun Zhang, Sylvie Bourassa, Elisabet Carlsohn, Jayson A. Falkner, Derek Smith, An Sung Chung, Sylvie Laboissiere, Helmut E. Meyer, Hong Wang, Jeffrey W. Smith, Hyoung Joo Lee, David A. Sarracino, Elmar Langenfeld, Kazuyuki Nakamura, Robert E. Kearney, Bing Yang, Bryan Krastins, Majlinda Kullolli |
| Rok vydání: |
2009 |
| Předmět: |
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| Zdroj: |
Nature methods
Biochemistry Publications |
| ISSN: |
1548-7105
1548-7091 |
| Popis: |
We carried out a test sample study to try to identify errors leading to irreproducibility, including incompleteness of peptide sampling, in LC-MS-based proteomics. We distributed a test sample consisting of an equimolar mix of 20 highly purified recombinant human proteins, to 27 laboratories for identification. Each protein contained one or more unique tryptic peptides of 1250 Da to also test for ion selection and sampling in the mass spectrometer. Of the 27 labs, initially only 7 labs reported all 20 proteins correctly, and only 1 lab reported all the tryptic peptides of 1250 Da. Nevertheless, a subsequent centralized analysis of the raw data revealed that all 20 proteins and most of the 1250 Da peptides had in fact been detected by all 27 labs. The centralized analysis allowed us to determine sources of problems encountered in the study, which include missed identifications (false negatives), environmental contamination, database matching, and curation of protein identifications. Improved search engines and databases are likely to increase the fidelity of mass spectrometry-based proteomics. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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