PSVI-18 Evaluation of alternative methodologies for dry matter degradability determination in the in vitro gas production method

Autor: Annelise Aila G Gomes Lobo, Rafaela Vincenzi, Vanderlei Benetel Junior, Leticia L Panosso, Yuli Andrea Pena Bermudez, Ives Cláudio da Silva Bueno, Richard R Lobo
Rok vydání: 2020
Předmět:
Zdroj: J Anim Sci
ISSN: 1525-3163
0021-8812
Popis: The use of filter crucibles to determine the degradability increases the time required in the analytical process, thus reducing the speed of obtaining experimental data. This has made it necessary to search for alternative methods that ensure a homogeneous digestion and filtration system for all samples and provide accurate and accurate results. The study evaluated the in vitro dry matter degradability of five treatments: 1) Filter crucible; 2) F57 bags (Ankom®) with non-degradable mass; 3) TNT bags weighing g/m2 with non-degradable mass; 4) F57 bags (Ankom®); 5) TNT bags weighing g/m2. The in vitro gas production method used was the semiautomatic one with pressure transducer, for that 8 bovines Nellore castrated male were used as content and ruminal liquid donors. A completely randomized experimental design was used, with 5 treatments, 4 blocks (inocula), and three replicates within each block. PROC GLM was used to adjust for multiple comparisons of the data using the Tukey’s test at 5% significance. There was no difference in the in vitro dry matter degradability for 24 or 96 hours (P-value 0.0554 and 0.1411, respectively), regardless of the treatment. Thus, the possibility of using TNT made bag to the detriment of the F57 bag is affirmed because of the high cost of this one in relation to that one.
Databáze: OpenAIRE