Impedance spectroscopy study of the retinal pigment epithelium: Application to the monitoring of blue light exposure effect on A2E-loaded in-vitro cell cultures

Autor: Agathe Alviset, Valérie Fradot, Olivier Français, Serge Picaud, Jocelyn Boutzen, Gaelle Lissorgues, Lionel Rousseau, Manon Valet
Přispěvatelé: Electronique, Systèmes de communication et Microsystèmes (ESYCOM), Université Paris-Est Marne-la-Vallée (UPEM)-ESIEE Paris-Conservatoire National des Arts et Métiers [CNAM] (CNAM), Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Centre National de la Recherche Scientifique (CNRS)-Université Gustave Eiffel, Institut de la Vision, Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Centre National de la Recherche Scientifique (CNRS)-Université Gustave Eiffel, ESIEE Paris, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), CCSD, Accord Elsevier, Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Paris-Est Marne-la-Vallée (UPEM)-ESIEE Paris, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)
Rok vydání: 2019
Předmět:
Light
[SPI] Engineering Sciences [physics]
[SDV]Life Sciences [q-bio]
Biomedical Engineering
Biophysics
Cell Culture Techniques
Impedance spectroscopy
02 engineering and technology
Electrolyte
Biosensing Techniques
Retinal Pigment Epithelium
01 natural sciences
A2E
[SPI.MAT]Engineering Sciences [physics]/Materials
[SPI]Engineering Sciences [physics]
Retinoids
Electrochemistry
medicine
Extracellular
Humans
[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics
[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials
ComputingMilieux_MISCELLANEOUS
Retinal pigment epithelium
Tight junction
Chemistry
Constant phase element
010401 analytical chemistry
General Medicine
021001 nanoscience & nanotechnology
Epithelium
0104 chemical sciences
Dielectric spectroscopy
[SPI.TRON]Engineering Sciences [physics]/Electronics
[SDV] Life Sciences [q-bio]
[SPI.ELEC]Engineering Sciences [physics]/Electromagnetism
medicine.anatomical_structure
Dielectric Spectroscopy
Electrode
0210 nano-technology
Blue light
Biotechnology
Zdroj: Biosensors and Bioelectronics
Biosensors and Bioelectronics, Elsevier, 2020, 161, pp.112180. ⟨10.1016/j.bios.2020.112180⟩
Biosensors and Bioelectronics, 2020, 161, pp.112180. ⟨10.1016/j.bios.2020.112180⟩
ISSN: 1873-4235
0956-5663
DOI: 10.1016/j.bios.2020.112180⟩
Popis: International audience; In age-related macular degeneration, the retinal pigment epithelium can be damaged by light acting on photosensitizers like N-retinylidene-N-retinylethanolamine (A2E). In this paper, the underlying cellular mechanism of lesion at the cell layer scale is analyzed by impedance spectroscopy. Retinal pigment epithelium (RPE) cells are cultured on top of custom-made electrodes capable of taking impedance measurements, with the help of a custom-made electronic setup but without the use of any chemical markers. An incubator is used to house the cells growing on the electrodes. An electrical model circuit is presented and linked to the constituents of the cell layer in which various electrical elements have been defined including a constant phase element (CPE) associated to the interface between the cell layer and the electrolyte. Their values are extracted from the fitted model of the measured impedance spectra. In this paper, we first investigate which parameters of the model can be analyzed independently. In that way, the parameter's evolution is examined with respect to two different targeted changes of the epithelium: 1. degradation of tight junctions between cells by extracellular calcium sequestration with Ethylenediaminetetraacetic acid (EDTA); 2. application of high amplitude short length electric field pulses. Based on the results obtained showing a clear relation between the model and the physiological state of the cell layer, the same procedure is applied to blue light exposure experiment. When A2E-loaded cells are exposed to blue light, the model parameters indicate, as expected, a clear degradation of the cell layer opposed to a relative stability of the not loaded ones.
Databáze: OpenAIRE
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