Snake Venom Identification via Fluorescent Discrimination
| Autor: | Wei Liu, Meng Qin, Wanjie Ren, Fengyu Li, Fei Chen, Fan Wang, Huihua Xu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
integumentary system
biology Chemistry digestive oral and skin physiology Phosphate buffered saline Venom Snakes complex mixtures Fluorescence Analytical Chemistry Sensor array Biochemistry Snake venom Clinical diagnosis biology.protein Animals Humans Bovine serum albumin Clinical treatment Snake Venoms |
| Zdroj: | Analytical chemistry. 93(42) |
| ISSN: | 1520-6882 |
| Popis: | The identification and discrimination of snake venom are highly desired for timely clinical treatment. However, the complex components in snake venom make it a great challenge to achieve rapid and accurate identification. Inspired by the organism's taste sensing system, a fluorescent sensor array that could differentiate snake venoms was fabricated. The interaction of snake venoms with different fluorescent dyes in the sensor array gave rich information, based on which efficient detection of complex snake venom was achieved. The main six proteins of snake venom in the same concentration, different concentrations, and their mixtures were identified with 100% accuracy. Furthermore, seven snake venoms belonging to different snake families were discriminated in PBS buffer and human plasma. Interferents of bovine serum albumin (BSA), thrombin, and transferrin (TRF) demonstrated the practicability of the fluorescent sensor array. This strategy of a multiresponse sensor array provides an effective method for accurate and rapid venom toxicology analysis, benefiting early and timely clinical diagnosis and treatment. |
| Databáze: | OpenAIRE |
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