Overexpression of heat shock GroEL stress protein in leptospiral biofilm
| Autor: | Kalimuthusamy Natarajaseenivasan, Paluru Vijayachari, R. Vimal Raj, K. Vinod Kumar, P. Surya, C. Kartick, Chandan Lall, K. Vedhagiri |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
animal diseases 030106 microbiology Immunoblotting Gene Expression Microbiology Zoonotic disease Fight-or-flight response 03 medical and health sciences Bacterial Proteins Leptospira GroEL Protein biology Biofilm Chaperonin 60 biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification GroEL Molecular biology Infectious Diseases Chaperone (protein) Biofilms Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization biology.protein bacteria |
| Zdroj: | Microbial pathogenesis. 102 |
| ISSN: | 1096-1208 |
| Popis: | Leptospira is the causative agent of leptospirosis, which is an emerging zoonotic disease. Recent studies on Leptospira have demonstrated biofilm formation on abiotic surfaces. The protein expressed in the biofilm was investigated by using SDS-PAGE and immunoblotting in combination with MALDI-TOF mass spectrometry. The proteins expressed in Leptospira biofilm and planktonic cells was analyzed and compared. Among these proteins, one (60 kDa) was found to overexpress in biofilm as compared to the planktonic cells. MALDI-TOF analysis identified this protein as stress and heat shock chaperone GroEL. Our findings demonstrate that GroEL is associated with Leptospira biofilm. GroEL is conserved, highly immunogenic and a prominent stress response protein in pathogenic Leptospira spp., which may have clinical relevance. |
| Databáze: | OpenAIRE |
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