The DNA sequence of the equine herpesvirus 4 gene encoding glycoprotein gp17/18, the homologue of herpes simplex virus glycoprotein gD
| Autor: | Andrew J. Davison, G. Allen, Ann Cullinane, L. Wilson, J. Neilan |
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| Rok vydání: | 1993 |
| Předmět: |
Genes
Viral Molecular Sequence Data Restriction Mapping Equine herpesvirus 1 medicine.disease_cause Open Reading Frames Viral Envelope Proteins Virology medicine Animals Simplexvirus Amino Acid Sequence Horses Gene Peptide sequence Herpesviridae Viral Structural Proteins chemistry.chemical_classification Base Sequence Sequence Homology Amino Acid biology Nucleic acid sequence biology.organism_classification Herpesvirus glycoprotein B Molecular biology Herpes simplex virus chemistry DNA Viral Equine herpesvirus Glycoprotein |
| Zdroj: | Journal of General Virology. 74:1959-1964 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-74-9-1959 |
| Popis: | The nucleotide sequence of the gene to the left of the gI gene of equine herpesvirus 4 (EHV-4) was determined. The gene encodes a peptide of 402 amino acids with an unprocessed M(r) of 45,323. The predicted polypeptide has several features of a glycoprotein including a hydrophobic signal sequence, a membrane spanning domain and four potential N-linked glycosylation sites within the proposed external domain. The predicted amino acid sequence of EHV-4 gD shows 83% identity with that of equine herpesvirus 1 gD. Conservation of the tertiary structure is suggested by the alignment of six cysteine residues with those of the gD of six other alphaherpesviruses. Screening a lambda gt11/EHV-4 expression library with monoclonal antibodies against several of the most abundant EHV-4 glycoproteins unequivocally identified the protein encoded by the EHV-4 gD gene as gp17/18. |
| Databáze: | OpenAIRE |
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