31P nuclear magnetic resonance measurements of phosphate metabolites and intracellular pH in turbotPsetta maximared blood cells using a novel flow method

Autor: M. T. Thebault, N. Kervarec, K. Pichavant
Přispěvatelé: Optimisation des régulations physiologiques (ORPHY (EA 4324)), Université de Brest (UBO)-Centre Hospitalier Régional Universitaire de Brest (CHRU Brest)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO)-Université de Brest (UBO), Service Commun RMN-RPE, Université de Brest (UBO)
Rok vydání: 2009
Předmět:
MESH: Hydrogen-Ion Concentration
Erythrocytes
Magnetic Resonance Spectroscopy
viruses
030310 physiology
Intracellular pH
Cell Respiration
Intracellular Space
MESH: Monitoring
Physiologic
Flow method
Aquatic Science
Phosphates
03 medical and health sciences
chemistry.chemical_compound
Nuclear magnetic resonance
Inorganic phosphate
[SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]
Pi
Animals
MESH: Animals
MESH: Phosphorus Isotopes
Ecology
Evolution
Behavior and Systematics
Monitoring
Physiologic
030304 developmental biology
0303 health sciences
biology
MESH: Magnetic Resonance Spectroscopy
MESH: Erythrocytes
Phosphorus Isotopes
Partial pressure
Hydrogen-Ion Concentration
Phosphate
biology.organism_classification
MESH: Flatfishes
3. Good health
Turbot
chemistry
MESH: Phosphates
Flatfishes
MESH: Intracellular Space
MESH: Cell Respiration
Nucleoside
Zdroj: Journal of Fish Biology
Journal of Fish Biology, Wiley, 2009, 75 (3), pp.747-54. ⟨10.1111/j.1095-8649.2009.02351.x⟩
ISSN: 1095-8649
0022-1112
DOI: 10.1111/j.1095-8649.2009.02351.x
Popis: International audience; A method for oxygenating and mixing suspensions of turbot Psetta maxima red blood cells (RBC) was tested in (31)P nuclear magnetic resonance (NMR) spectroscopy. In normoxia, the levels of inorganic phosphate (Pi) and nucleoside triphosphates (NTP) were stable up to 140 min and intracellular pH (pHi) was maintained and decreased oxygen partial pressure (P(O ( 2) )) from 30 to 15 and 600 Pa produced a significant fall in the intensity of NTP resonance, balanced by an increase in the Pi signal. Treatment of RBC with 0. 5 M isoproterenol during hypoxia exposure did not affect the pattern of changes in NTP or pHi induced by hypoxia and the effect was manifest only on Pi levels.
Databáze: OpenAIRE
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