Correction: Identification of a Tumor Specific, Active-Site Mutation in Casein Kinase 1α by Chemical Proteomics
| Autor: | Eric Okerberg, John W. Kozarich, Ann Shih, Tyzoon K. Nomanbhoy, Jane Wu, Arwin Aban, Heidi E. Brown, Anna Hainley, Matthew P. Patricelli, Senait Alemayehu, Jonathan S. Rosenblum |
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| Rok vydání: | 2017 |
| Předmět: |
Proteomics
Colon Molecular Sequence Data Mutation Missense Tumor specific lcsh:Medicine Computational biology Adenocarcinoma Biology Text mining Catalytic Domain Humans lcsh:Science Multidisciplinary Base Sequence business.industry lcsh:R Correction Casein Kinase Ialpha Active site HEK293 Cells Colonic Neoplasms Mutation (genetic algorithm) biology.protein lcsh:Q Identification (biology) Casein kinase 1 business |
| Zdroj: | PLoS ONE, Vol 12, Iss 2, p e0172649 (2017) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0172649 |
| Popis: | We describe the identification of a novel, tumor-specific missense mutation in the active site of casein kinase 1α (CSNK1A1) using activity-based proteomics. Matched normal and tumor colon samples were analyzed using an ATP acyl phosphate probe in a kinase-targeted LC-MS2 platform. An anomaly in the active-site peptide from CSNK1A1 was observed in a tumor sample that was consistent with an altered catalytic aspartic acid. Expression and analysis of the suspected mutant verified the presence of asparagine in the probe-labeled, active-site peptide for CSNK1A1. Genomic sequencing of the colon tumor samples confirmed the presence of a missense mutation in the catalytic aspartic acid of CSNK1A1 (GAC→AAC). To our knowledge, the D163N mutation in CSNK1A1 is a newly defined mutation to the conserved, catalytic aspartic acid of a protein kinase and the first missense mutation identified using activity-based proteomics. The tumorigenic potential of this mutation remains to be determined. |
| Databáze: | OpenAIRE |
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