Sterol enriched fractions obtained from Agaricus bisporus fruiting bodies and by-products by compressed fluid technologies (PLE and SFE)
| Autor: | Guillermo Reglero, Marimuthu Palanisamy, Alicia Gil-Ramírez, Alejandro Ruiz-Rodríguez, Laila Aldars-García, Francisco R. Marín, Rodica M. Jiverdeanu, Cristina Soler-Rivas |
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| Přispěvatelé: | UAM. Departamento de Química Física Aplicada |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
2. Zero hunger
Supercritical CO2 extraction Chemistry Emerging technologies business.industry 010401 analytical chemistry 04 agricultural and veterinary sciences General Chemistry Accelerated solvent extraction (ASE) Química 040401 food science 01 natural sciences Industrial and Manufacturing Engineering 0104 chemical sciences 0404 agricultural biotechnology Cholesterol Button mushroom Ergosterol Botany Process engineering business Agaricus bisporus Food Science |
| Zdroj: | Biblos-e Archivo. Repositorio Institucional de la UAM instname |
| Popis: | This is the author’s version of a work that was accepted for publication in Innovative Food Science and Emerging Technologies. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Innovative Food Science and Emerging Technologies, 18, (2013) DOI: 10.1016/j.ifset.2013.01.007 Ergosterol, ergosta7,22 dienol, ergosta 5,7 dienol, fungisterol, ergosta-4,7,2-trien-3-ona and ergosta-4,6,8(14),22-tetraen-3-ona were the fungal sterols detected in Agaricus bisporus mushrooms after optimization of a sterol extraction method. Their concentration ranged from 3.1 to 11.2 mg/g dw depending on the strain, casing soil, flush number, developmental stage and sporophore tissue analyzed. Two methods were optimized to obtain sterol enriched extracts from A. bisporus fruiting bodies using pressurized liquid extraction (PLE) and supercritical fluid extraction (SFE). PLE using ethanol as solvent at 10.7 MPa (50 C and 100 C) after 5 cycles of 5 min extraction (mixing in the extraction cell the sample with sand in a ratio 1:4) yielded extracts with respectively 5 and 2.9% sterols. Using SFE-CO2 at 40 C and 9 to 30 MPa fractions containing 60% of sterols were obtained. Both technologies could be also utilized to extract sterols from mushroom by-products (the lower part of the stipe) as a method for their valorization. Industrial relevance In this work, two environmentally friendly methods (SFE and PLE) to obtain sterol enriched fractions from Agaricus bisporus mushrooms were optimized. Extractions from both the complete fruiting body and the lower part of the stipe (usually discarded as a by-product during harvesting) yielded extracts with high ergosterol (and derivatives) content that could be used as functional ingredients to design novel foods with hypocholesterolemic properties since fungal sterols are able to reduce cholesterol levels in serum as plant phytosterols. Moreover, if the mushroom by-products are utilized as starting material, this application can be an interesting alternative method for the commercial valorization of this residue The research was supported by the European Union within the 7th Framework Program (7FM-PEOPLE-2009-IIF project 251285), ALIBIRDCMS2009/ AGR-1469 Regional Programfromthe Community ofMadrid (Spain) and AGL2010-21537 National R+D Program from the Spanish Ministry of Science and Innovation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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