Seminal Plasma Protein N-Glycan Peaks Are Potential Predictors of Semen Pathology and Sperm Chromatin Maturity in Men

Autor:	Iwona Wojcik, Ana Katušić Bojanac, Davor Jezek, Gordan Lauc, Irena Trbojević-Akmačić, Tea Petrović, Marcello Ceppi, Ana Matijevic, Aleksandra Fučić, Tihana Maric, Evangelini Evgeni, Marco Bruzzone
Rok vydání:	2021
Předmět:	Science Halosperm Semen DNA fragmentation N-glycosylation Biology aniline blue sperm General Biochemistry Genetics and Molecular Biology Article male infertility Male infertility Andrology medicine Ecology Evolution Behavior and Systematics Sperm motility seminal plasma sperm chromatin maturity Paleontology Environmental exposure medicine.disease Blood proteins Sperm Chromatin Space and Planetary Science
Zdroj:	Life Life, Vol 11, Iss 989, p 989 (2021) Volume 11 Issue 9
ISSN:	2075-1729
Popis:	Background: Male infertility is increasingly becoming a health and demographic problem. While it may originate from congenital or acquired diseases, it can also result from environmental exposure. Hence, the complexity of involved molecular mechanisms often requires a multiparametric approach. This study aimed to associate semen parameters with sperm DNA fragmentation, chromatin maturity and seminal plasma protein N-glycosylation. Methods: The study was conducted with 166 participants, 20–55 y old, 82 normozoospermic and 84 with pathological diagnosis. Sperm was analyzed by Halosperm assay and aniline blue staining, while seminal plasma total protein N-glycans were analyzed by ultra-high-performance liquid chromatography. Results: Sperm DNA fragmentation was significantly increased in the pathological group and was inversely correlated with sperm motility and viability. Seminal plasma total protein N-glycans were chromatographically separated in 37 individual peaks. The pattern of seminal plasma N-glycan peaks (SPGP) showed that SPGP14 significantly differs between men with normal and pathological semen parameters (p < 0.001). The multivariate analysis showed that when sperm chromatin maturity increases by 10%, SPGP17 decreases by 14% while SPGP25 increases by 25%. Conclusion: DNA integrity and seminal plasma N-glycans are associated with pathological sperm parameters. Specific N-glycans are also associated with sperm chromatin maturity and have a potential in future fertility research and clinical diagnostics.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4993a0dfe2dafae2f0ab7ad76e8517b https://pubmed.ncbi.nlm.nih.gov/34575138 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.