Knockdown of RRS1 by lentiviral-mediated RNAi promotes apoptosis and suppresses proliferation of human hepatocellular carcinoma cells
| Autor: | Zhi Li, Qingfan Xie, Baijun Sun, Jitao Wang, Ruizhao Qi, Dengxiang Liu, Zhongguang Zhen, Hui Li, Zhiwei Li, Changzeng Zuo, Junhong Jia |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Cancer Research Carcinoma Hepatocellular proliferation Cell Biology regulator of ribosome synthesis 1 03 medical and health sciences 0302 clinical medicine RNA interference Cell Line Tumor medicine Gene silencing Humans RNA Messenger Cell Proliferation Gene knockdown Cell growth Lentivirus Liver Neoplasms apoptosis Nuclear Proteins RNA-Binding Proteins General Medicine Transfection Articles hepatocellular carcinoma Cell cycle Cell biology Gene Expression Regulation Neoplastic 030104 developmental biology medicine.anatomical_structure Oncology Cell culture 030220 oncology & carcinogenesis Gene Knockdown Techniques Cancer research Female |
| Zdroj: | Oncology Reports |
| ISSN: | 1791-2431 1021-335X |
| Popis: | In recent years it was found that the synthesis and biological activity of ribosomes are closely associated with tumor cell growth, tumorigenesis, and malignant transformation. However, the role of regulator of ribosome synthesis 1 (RRS1) in hepatocellular carcinoma (HCC) has not yet been reported. In the present study, we aimed to examine the potential role of RRS1 in tumor cell growth by using a lentivirus-mediated RNA interference (RNAi) system in the HCC cell line SMMC-7721 in vitro. Compared with that of the negative control group (Lv-shCon), the mRNA and protein expression levels of RRS1 in SMMC-7721 cells transfected with Lv-shRRS1 were significantly decreased. Further experiments found that silencing of RRS1 gene expression in SMMC-7721 cells significantly suppressed cell proliferation, inhibited colony formation capacity, increased apoptosis and arrested cells in the G1 phase. These results suggest that the RRS1 gene plays a critical role in cell proliferation, colony formation, cell apoptosis and cell cycle distribution in human HCC cells, and that silencing of RRS1 by RNAi is a promising therapeutic approach for the treatment of HCC, and should be further developed. |
| Databáze: | OpenAIRE |
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