miR-96-5p, miR-134-5p, miR-181b-5p and miR-200b-3p heterogenous expression in sites of prostate cancer versus benign prostate hyperplasia—archival samples study
Autor: | Tomasz M. Grzywa, Filip Garbicz, Kinga Szczepaniak, Klaudia Klicka, Kacper Pełka, Janina M Marczewska, Agata Gondek, Wiktor Paskal, Paweł Włodarski |
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Rok vydání: | 2020 |
Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty Histology Prostatic Hyperplasia 03 medical and health sciences Prostate cancer 0302 clinical medicine Benign prostate hyperplasia Prostate microRNA medicine Humans Molecular Biology Aged Laser capture microdissection Original Paper business.industry Prostatic Neoplasms Cancer Cell Biology Middle Aged Hyperplasia medicine.disease MicroRNAs Medical Laboratory Technology 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Mir 200c business Benign prostate |
Zdroj: | Histochemistry and Cell Biology |
ISSN: | 1432-119X 0948-6143 |
Popis: | MicroRNAs are involved in various pathologies including cancer. The aim of the study was to assess the level of expression of miR-96-5p, -134-5p, -181b-5p, -200b-3p in FFPE samples of prostate cancer, adjacent cancer-free tissue, and benign prostatic hyperplasia. Samples of 23 FFPE prostate cancer and 22 benign prostatic hyperplasias were dissected and HE stained. Compartments of tumor tissue and adjacent healthy glandular tissue were isolated from each sample using Laser Capture Microdissection. Total RNA was isolated from dissected tissues. Expression of miR-96-5p, miR-134-5p, 181b-5p, and miR-200b-3p was determined by real-time RT-qPCR method. The expression of miR-200b-3p was significantly higher in cancerous prostate: both in adenocarcinomatous glands and in the adjacent, apparently unaffected glands compared to BPH samples. The expression of miR-181b-5p was lower in in both prostate cancer tissues and adjacent tissue compared to BPH samples. Expression of miR-96-5p and miR-134-5p was lower in prostate cancer tissues compared to BPH. Levels of miR-96-5p, miR-134-5p, and 181b-5p negatively correlated with the Gleason score. Given further studies, miR-96-5p, miR-134-5p and especially miR-200b-3p and miR-181b-5p may differentiate BPH and PC. Electronic supplementary material The online version of this article (10.1007/s00418-020-01941-2) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
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