Genetic diversity among multidrug-resistant Mycobacterium tuberculosis strains in Mexico

Autor: Alejandro Escobar-Gutiérrez, Sandra Rivera-Gutierrez, Gilberto Vaughan, Arely Vergara-Castañeda, Livia Maria Gonçalves Rossi, Nalin Rastogi, Jorge A. Gonzalez-y-Merchand, Daniela Lozano, David Couvin, Carlos A. Vazquez-Chacon, Armando Martinez-Guarneros
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Adult
DNA
Bacterial
Male
Microbiology (medical)
Tuberculosis
Adolescent
Genotype
medicine.drug_class
Antibiotics
Microbial Sensitivity Tests
Minisatellite Repeats
Microbiology
Mycobacterium tuberculosis
Young Adult
03 medical and health sciences
0302 clinical medicine
Tuberculosis
Multidrug-Resistant
Genetic variation
Genetics
medicine
Cluster Analysis
Humans
Multidrug-Resistant Mycobacterium tuberculosis
030212 general & internal medicine
Genetic variability
Child
Antibiotics
Antitubercular
Mexico
Molecular Biology
Ecology
Evolution
Behavior and Systematics
Aged
Aged
80 and over
0303 health sciences
Genetic diversity
biology
030306 microbiology
Genetic Variation
Middle Aged
biology.organism_classification
medicine.disease
Virology
3. Good health
Molecular Typing
Infectious Diseases
Child
Preschool
Female
Topography
Medical
Zdroj: Infection, Genetics and Evolution; Vol 14
ISSN: 1567-1348
DOI: 10.1016/j.meegid.2012.12.024
Popis: Tuberculosis is an important public health problem in Mexico. However, limited information about the genetic diversity of Mycobacterium tuberculosis strains circulating in the country is available. In this work, 109 multidrug-resistant (MDR) M. tuberculosis isolates collected in 23 different states of Mexico in 2003 were retrospectively characterized by spoligotyping and MIRU-VNTRs. All isolates, except for a single cluster containing two strains (subcluster E1), were split when information from the 12-loci MIRUs and spoligo-pattern was simultaneously analyzed. The discriminative power of 12-loci MIRU-VNTR and spoligotyping, by the Hunter-Gaston index, were 0.9998 and 0.9011, respectively. These findings suggest that almost all cases were epidemiologically unrelated. Instead, the genetic variations observed among these strains are suggestive of emergence of acquired drug-resistance during the course of treatment. The results suggest a high degree of genetic variability and a high frequency of SIT53 (T1 family) spoligotype among the MDR M. tuberculosis isolates included in the study.
Databáze: OpenAIRE
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