Genetic diversity among multidrug-resistant Mycobacterium tuberculosis strains in Mexico
Autor: | Alejandro Escobar-Gutiérrez, Sandra Rivera-Gutierrez, Gilberto Vaughan, Arely Vergara-Castañeda, Livia Maria Gonçalves Rossi, Nalin Rastogi, Jorge A. Gonzalez-y-Merchand, Daniela Lozano, David Couvin, Carlos A. Vazquez-Chacon, Armando Martinez-Guarneros |
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Jazyk: | angličtina |
Rok vydání: | 2013 |
Předmět: |
Adult
DNA Bacterial Male Microbiology (medical) Tuberculosis Adolescent Genotype medicine.drug_class Antibiotics Microbial Sensitivity Tests Minisatellite Repeats Microbiology Mycobacterium tuberculosis Young Adult 03 medical and health sciences 0302 clinical medicine Tuberculosis Multidrug-Resistant Genetic variation Genetics medicine Cluster Analysis Humans Multidrug-Resistant Mycobacterium tuberculosis 030212 general & internal medicine Genetic variability Child Antibiotics Antitubercular Mexico Molecular Biology Ecology Evolution Behavior and Systematics Aged Aged 80 and over 0303 health sciences Genetic diversity biology 030306 microbiology Genetic Variation Middle Aged biology.organism_classification medicine.disease Virology 3. Good health Molecular Typing Infectious Diseases Child Preschool Female Topography Medical |
Zdroj: | Infection, Genetics and Evolution; Vol 14 |
ISSN: | 1567-1348 |
DOI: | 10.1016/j.meegid.2012.12.024 |
Popis: | Tuberculosis is an important public health problem in Mexico. However, limited information about the genetic diversity of Mycobacterium tuberculosis strains circulating in the country is available. In this work, 109 multidrug-resistant (MDR) M. tuberculosis isolates collected in 23 different states of Mexico in 2003 were retrospectively characterized by spoligotyping and MIRU-VNTRs. All isolates, except for a single cluster containing two strains (subcluster E1), were split when information from the 12-loci MIRUs and spoligo-pattern was simultaneously analyzed. The discriminative power of 12-loci MIRU-VNTR and spoligotyping, by the Hunter-Gaston index, were 0.9998 and 0.9011, respectively. These findings suggest that almost all cases were epidemiologically unrelated. Instead, the genetic variations observed among these strains are suggestive of emergence of acquired drug-resistance during the course of treatment. The results suggest a high degree of genetic variability and a high frequency of SIT53 (T1 family) spoligotype among the MDR M. tuberculosis isolates included in the study. |
Databáze: | OpenAIRE |
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