Molecular mechanisms of nanosized titanium dioxide-induced pulmonary injury in mice
| Autor: | Ling Wang, Xiaochun Wang, Qingqing Sun, Meng Tang, Ting-Ting Zhang, Danlin Tan, Bing Li, Suxin Gui, Yaling Cui, Xuezi Sang, Min Zhu, Yuguan Ze, Xiaoyang Zhao, Lei Sheng, Fashui Hong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Mouse
Metal Nanoparticles Gene Expression lcsh:Medicine medicine.disease_cause Biochemistry chemistry.chemical_compound Mice Molecular Cell Biology lcsh:Science Lung Titanium Alloys Oligonucleotide Array Sequence Analysis chemistry.chemical_classification Titanium Multidisciplinary Microscopy Confocal Cell Death Enzyme Classes Animal Models Cell cycle Cell biology Up-Regulation Enzymes Chemistry Metallurgy Signal transduction medicine.symptom Bronchoalveolar Lavage Fluid Research Article Immunology Down-Regulation Inflammation Biology Real-Time Polymerase Chain Reaction Metal Alloys Model Organisms Lactate dehydrogenase medicine Genetics Animals Dehydrogenases Reactive oxygen species Cell growth Gene Expression Profiling lcsh:R technology industry and agriculture Immunologic Subspecialties Molecular biology Oxidative Stress chemistry Apoptosis lcsh:Q Lipid Peroxidation Reactive Oxygen Species Pulmonary Immunology Oxidative stress |
| Zdroj: | PLoS ONE, Vol 8, Iss 2, p e55563 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The pulmonary damage induced by nanosized titanium dioxide (nano-TiO2) is of great concern, but the mechanism of how this damage may be incurred has yet to be elucidated. Here, we examined how multiple genes may be affected by nano-TiO2 exposure to contribute to the observed damage. The results suggest that long-term exposure to nano-TiO2 led to significant increases in inflammatory cells, and levels of lactate dehydrogenase, alkaline phosphate, and total protein, and promoted production of reactive oxygen species and peroxidation of lipid, protein and DNA in mouse lung tissue. We also observed nano-TiO2 deposition in lung tissue via light and confocal Raman microscopy, which in turn led to severe pulmonary inflammation and pneumonocytic apoptosis in mice. Specifically, microarray analysis showed significant alterations in the expression of 847 genes in the nano-TiO2-exposed lung tissues. Of 521 genes with known functions, 361 were up-regulated and 160 down-regulated, which were associated with the immune/inflammatory responses, apoptosis, oxidative stress, the cell cycle, stress responses, cell proliferation, the cytoskeleton, signal transduction, and metabolic processes. Therefore, the application of nano-TiO2 should be carried out cautiously, especially in humans. |
| Databáze: | OpenAIRE |
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