Evaluation of cynomolgus macaque (Macaca fascicularis) endogenous retrovirus expression following simian immunodeficiency virus infection
| Autor: | Oluwadamilola H. Iwajomo, Jacqueline K. Chan, Angie K. Marsh, Kelly S. MacDonald, Olena Skokovets, David O. Willer |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Genes
Viral animal diseases viruses Simian Acquired Immunodeficiency Syndrome Gene Expression Endogenous retrovirus lcsh:Medicine Macaque 0302 clinical medicine Molecular Cell Biology Gene expression HIV vaccine lcsh:Science 0303 health sciences Multidisciplinary biology Vaccination virus diseases Animal Models Viral Load Immunizations 3. Good health 030220 oncology & carcinogenesis Simian Immunodeficiency Virus Disease Susceptibility Viral load Research Article Gene Expression Regulation Viral Immunology Molecular Sequence Data Down-Regulation Gene Products gag Microbiology Peripheral blood mononuclear cell Immune Activation 03 medical and health sciences Model Organisms Downregulation and upregulation Virology biology.animal Retroviruses Animals Humans Amino Acid Sequence RNA Messenger Biology Immunity to Infections 030304 developmental biology Endogenous Retroviruses lcsh:R Immunity Viral Vaccines CD4 Lymphocyte Count Animal Models of Infection Macaca fascicularis Viral Classification Leukocytes Mononuclear lcsh:Q Sequence Alignment |
| Zdroj: | PLoS ONE, Vol 7, Iss 6, p e40158 (2012) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Human endogenous retrovirus type K (HERV-K) transcripts are upregulated in the plasma of HIV-infected individuals and have been considered as targets for an HIV vaccine. We evaluated cynomolgus macaque endogenous retrovirus (CyERV) mRNA expression by RT-qPCR in PBMCs isolated from a cohort of animals previously utilized in a live attenuated SIV vaccine trial. CyERV env transcript levels decreased following vaccination (control and vaccine groups) and CyERV env and gag mRNA expression was decreased following acute SIV-infection, whereas during chronic SIV infection, CyERV transcript levels were indistinguishable from baseline. Reduced susceptibility to initial SIV infection, as measured by the number of SIV challenges required for infection, was associated with increased CyERV transcript levels in PBMCs. In vitro analysis revealed that SIV infection of purified CD4(+) T-cells did not alter CyERV gene expression. This study represents the first evaluation of ERV expression in cynomolgus macaques following SIV infection, in an effort to assess the utility of cynomolgus macaques as an animal model to evaluate ERVs as a target for an HIV/SIV vaccine. This non-human primate model system does not recapitulate what has been observed to date in the plasma of HIV-infected humans suggesting that further investigation at the cellular level is required to elucidate the impact of HIV/SIV infection on endogenous retrovirus expression. |
| Databáze: | OpenAIRE |
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