Effects of Srxn1 on growth and Notch signalling of astrocyte induced by hydrogen peroxide
| Autor: | Lei Yu, Changwei Ni, Guangjun Lin, Huizi Gu, Lan Li |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Biomedical Engineering
Notch signaling pathway Pharmaceutical Science Medicine (miscellaneous) Apoptosis 02 engineering and technology medicine.disease_cause Flow cytometry Superoxide dismutase Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Western blot Malondialdehyde medicine Animals Oxidoreductases Acting on Sulfur Group Donors Notch 1 Cell Proliferation biology medicine.diagnostic_test L-Lactate Dehydrogenase Receptors Notch Chemistry Superoxide Dismutase General Medicine Hydrogen Peroxide 021001 nanoscience & nanotechnology Cell biology Rats Oxidative Stress medicine.anatomical_structure Animals Newborn Gene Expression Regulation 030220 oncology & carcinogenesis Astrocytes Gene Knockdown Techniques biology.protein 0210 nano-technology Oxidative stress Biotechnology Astrocyte Signal Transduction |
| Zdroj: | Artificial cells, nanomedicine, and biotechnology. 47(1) |
| ISSN: | 2169-141X |
| Popis: | Objective To investigate the effect of Sulfiredoxin-1 (Srxn1) on astrocyte injury induced by hydrogen peroxide (H2O2). Methods Observing the changes of H2O2 on contents of lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD) and apoptosis after transfected Srxn1 siRNA into astrocytes. The protein expression of Notch 1, NICD and Hes1, the content of LDH and MDA, the activity of SOD and apoptosis rate of astrocytes after inhibiting or activation of Notch signalling pathway were detected by Western blot, ELISA and flow cytometry, respectively. Results Knockdown of Srxn1 could promote the secretion of LDH and MDA, decrease the activity of SOD and aggravate apoptosis of astrocytes induced by H2O2. The results of Western blot, ELISA assay and flow cytometry indicated that activation of the Notch signalling pathway attenuated the effect of Srxn1 on H2O2-induced oxidative damage and apoptosis of astrocytes. Conclusion Srxn1 may protect astrocytes from oxidative stress injury induced by H2O2 by activation of Notch signalling pathway. |
| Databáze: | OpenAIRE |
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