Structure and dynamics of the pan-genome of Streptococcus pneumoniae and closely related species
| Autor: | Rino Rappuoli, Alessandro Muzzi, Claudio Donati, Marco R. Oggioni, Timothy J. Mitchell, Garth D. Ehrlich, Fen Z. Hu, Antonello Covacci, Stephen D. Bentley, E. Richard Moxon, Hervé Tettelin, Morgens Kilian, David R. Riley, Samuel V. Angiuoli, Julie C. Dunning Hotopp, Vega Masignani, Nicholas J. Croucher, N. Luisa Hiller |
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| Přispěvatelé: | Donati C, Hiller NL, Tettelin H, Muzzi A, Croucher NJ, Angiuoli SV, Oggioni M, Dunning Hotopp JC, Hu FZ, Riley DR, Covacci A, Mitchell TJ, Bentley SD, Kilian M, Ehrlich GD, Rappuoli R, Moxon ER, Masignani V |
| Jazyk: | angličtina |
| Předmět: |
DNA
Bacterial Gene Conversion Microbiologia Virulence Streptococcus mitis medicine.disease_cause Polymorphism Single Nucleotide Genome Linkage Disequilibrium Evolution Molecular 03 medical and health sciences Phylogenetics Bioinformatica Streptococcus pneumoniae medicine Gene Phylogeny 030304 developmental biology Genetics 0303 health sciences Streptococcus pseudopneumoniae biology 030306 microbiology Research Genetic Variation Pan-genome Sequence Analysis DNA biology.organism_classification respiratory tract diseases Genes Bacterial Multigene Family FOS: Biological sciences Genomica Sequence Alignment human activities Genome Bacterial 69999 Biological Sciences not elsewhere classified |
| Zdroj: | Genome Biology Genome Biology; Vol 11 Donati, C, Hiller, N L, Tettelin, H, Muzzi, A, Croucher, N J, Angiuoli, S V, Oggioni, M, Dunning Hotopp, J C, Hu, F Z, Riley, D R, Covacci, A, Mitchell, T J, Bentley, S D, Kilian, M, Ehrlich, G D, Rappuoli, R, Moxon, E R & Masignani, V 2010, ' Structure and dynamics of the pan-genome of Streptococcus pneumoniae and closely related species ', Genome Biology, vol. 11, no. 10, pp. R107 . https://doi.org/10.1186/gb-2010-11-10-r107 |
| ISSN: | 1465-6906 |
| DOI: | 10.1186/gb-2010-11-10-r107 |
| Popis: | BACKGROUND: Streptococcus pneumoniae is one of the most important causes of microbial diseases in humans. The genomes of 44 diverse strains of S. pneumoniae were analyzed and compared with strains of non-pathogenic streptococci of the Mitis group. RESULTS: Despite evidence of extensive recombination, the S. pneumoniae phylogenetic tree revealed six major lineages. With the exception of serotype 1, the tree correlated poorly with capsular serotype, geographical site of isolation and disease outcome. The distribution of dispensable genes - genes present in more than one strain but not in all strains - was consistent with phylogeny, although horizontal gene transfer events attenuated this correlation in the case of ancient lineages. Homologous recombination, involving short stretches of DNA, was the dominant evolutionary process of the core genome of S. pneumoniae. Genetic exchange occurred both within and across the borders of the species, and S. mitis was the main reservoir of genetic diversity of S. pneumoniae. The pan-genome size of S. pneumoniae increased logarithmically with the number of strains and linearly with the number of polymorphic sites of the sampled genomes, suggesting that acquired genes accumulate proportionately to the age of clones. Most genes associated with pathogenicity were shared by all S. pneumoniae strains, but were also present in S. mitis, S. oralis and S. infantis, indicating that these genes are not sufficient to determine virulence. CONCLUSIONS: Genetic exchange with related species sharing the same ecological niche is the main mechanism of evolution of S. pneumoniae. The open pan-genome guarantees the species a quick and economical response to diverse environments. |
| Databáze: | OpenAIRE |
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