Characterization of polysaccharides extracted from Platycodon grandiflorus (Jacq.) A.DC. affecting activation of chicken peritoneal macrophages
| Autor: | Jianzhu Liu, Shenghua Wang, Pimiao Zheng, Huiyu Wan, Pan Hao, Zhihui Hao, Yang Wang, Xiaona Zhao, Wentao Fan |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Arabinose Platycodon Rhamnose Mannose 02 engineering and technology Platycodon grandiflorus Xylose Polysaccharide Nitric Oxide Biochemistry Immunomodulation 03 medical and health sciences chemistry.chemical_compound Structural Biology Polysaccharides Animals Molecular Biology Cell Proliferation CD86 chemistry.chemical_classification biology Monosaccharides General Medicine Macrophage Activation 021001 nanoscience & nanotechnology biology.organism_classification Molecular Weight 030104 developmental biology chemistry Galactose B7-1 Antigen Macrophages Peritoneal Cytokines B7-2 Antigen 0210 nano-technology Chickens |
| Zdroj: | International journal of biological macromolecules. 96 |
| ISSN: | 1879-0003 |
| Popis: | Polysaccharides were isolated from Platycodon grandiflorus (Jacq.) A.DC. (PG) and the effects of three polysaccharides (PGPS80, PGPS60, PGPSt) on their immunological activities were studied. The structure identification of PGPSs was assessed using physicochemical and spectral methods. Results showed that PGPSt(2.67×105Da) compared to PGPS80(1.01×105Da) and PGPS60(1.12×105Da) has relatively higher average molecular weight(Mw) at the first peak with a narrower molecular weight distribution and all consisted of glucose, mannose, arabinose, galactose, xylose and rhamnose in different mass percentages. PGPS80 and PGPSt linked mainly by 1,3-and 1,6-β-d-Galp residues. The immunological efficacy of PGPSs was performed on chicken peritoneal macrophages. Results showed that PGPSt significantly increased phagocytic rates, proliferation and NO production, stimulated macrophages to produce cytokines, including TNF-α, IL-1β and IL-6 as well as stimulated macrophages to express the maturation markers CD80 and CD86. These findings suggest that PGPSt exerted significant immunological activity and might be associated with special characters. |
| Databáze: | OpenAIRE |
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