Polymerase Chain Reaction Human Papillomavirus (HPV) Detection and HPV Genotyping in Invasive Cervical Cancers With Prior Negative HC2 Test Results
| Autor: | Zhengyu Zeng, Christopher C. Griffith, Baowen Zheng, Zaibo Li, Bing Luo, Xiang Tao, Fu-Fen Yin, Xianrong Zhou, Xiangdong Ding, Chengquan Zhao |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
0301 basic medicine Oncology medicine.medical_specialty Genotype Hpv genotyping Uterine Cervical Neoplasms Polymerase Chain Reaction law.invention 03 medical and health sciences 0302 clinical medicine law Cytology Internal medicine Humans Medicine Human papillomavirus False Negative Reactions Papillomaviridae Genotyping Early Detection of Cancer Polymerase chain reaction Vaginal Smears Cervical cancer business.industry Papillomavirus Infections General Medicine Middle Aged medicine.disease 030104 developmental biology 030220 oncology & carcinogenesis DNA Viral Female business Cohort study |
| Zdroj: | American Journal of Clinical Pathology. 147:477-483 |
| ISSN: | 1943-7722 0002-9173 |
| DOI: | 10.1093/ajcp/aqx027 |
| Popis: | Objectives Recently, three large Chinese cohort studies showed that 7.5% to 15.5% of patients with cervical carcinoma had negative high-risk human papillomavirus (hrHPV) test results on prior cytology specimens. These studies raise the question as to whether these negative hrHPV results represent truly hrHPV-negative carcinomas or false-negative hrHPV test results due to limitations in the testing of cytology specimens. This is increasingly an important question with increasing push to use hrHPV testing alone to screen for cervical cancer. Methods We investigated the hrHPV status on the surgical specimens from these same patients with cervical carcinoma using three polymerase chain reaction methods and a linear assay genotyping method. Results A variety of HPV genotypes were detected in 28 (45.9%) of 61 cases, all belonging to carcinogenic or possibly carcinogenic categories. HPV 16 was the most common genotype detected in positive cases (66.7%). HPV was detected in 25 (50%) of 50 squamous cell carcinomas and two (66.7%) of three adenosquamous carcinomas and only one (12.5%) of eight endocervical adenocarcinomas. Conclusions Our findings demonstrate that negative hrHPV testing on cervicovaginal cytology specimens in women later diagnosed with cervical carcinoma can be explained by the occurrence of truly HPV-negative carcinomas in more than half of patients. These results should be considered in the development of future cervical cancer screening guidelines. |
| Databáze: | OpenAIRE |
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