Sanger and next generation sequencing in the characterisation of arbuscular mycorrhizal fungi (AMF) in Pancratium maritimum L. (Amaryllidaceae), a representative plant species of Mediterranean sand dunes
| Autor: | Marco Guida, Antonietta Di Maio, Mariano Avino, Bruno Menale, Olga De Castro |
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| Přispěvatelé: | De Castro, Olga, Avino, Mariano, Di Maio, Antonietta, Menale, Bruno, Guida, Marco |
| Rok vydání: | 2018 |
| Předmět: |
SSU–ITS–LSU
0106 biological sciences 0301 basic medicine Pancratium maritimum ITS2 Plant Science DNA Ribosomal Plant Roots 01 natural sciences DNA barcoding DNA sequencing 03 medical and health sciences symbols.namesake Genetic Mycorrhizae Sea daffodil Botany Genetics DNA Barcoding Taxonomic Colonization DNA Fungal Sanger sequencing Massive parallel sequencing biology Amaryllidaceae Fungi High-Throughput Nucleotide Sequencing Sequence Analysis DNA Illumina MiSeq Ribosomal RNA biology.organism_classification Psammophilic plant 030104 developmental biology Root symbols 010606 plant biology & botany |
| Zdroj: | Planta. 248:1443-1453 |
| ISSN: | 1432-2048 0032-0935 |
| Popis: | Main conclusion An interesting AMF colonization microcosm has been detected in the roots of Pancratium maritimum (sea daffodil). Both sequencing techniques (Sanger and NGS) have been used for AMF characterisation, showing a balanced trade-off between pros and cons. By Sanger and next generation sequencing of rRNA nuclear molecular markers (SSU–ITS–LSU and ITS2, respectively), the presence of AMF communities in the roots of P. maritimum was evaluated. Our results shed light on the presence of AMF in sea daffodil and the diversity of assemblages of AMF detected after Sanger sequencing of the SSU–ITS–LSU marker is much higher than that determined following NGS sequencing of ITS2 alone. |
| Databáze: | OpenAIRE |
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