Impaired spermatogenesis caused by busulfan is partially ameliorated by treatment with conditioned medium of adipose tissue derived mesenchymal stem cells
| Autor: | Fatemeh Fadaei Fathabadi, Abbas Aliaghaei, Mohammad-Amin Abdollahifar, Faezeh Ghanimat, Hojjat-Allah Abbaszadeh, Vahid Ebrahimi, Amir Raoofi, Maryam Movahedi, Shabnam Abdi, Nahid Azad, Maryam Faraji Sani |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Histology Adipose tissue Motility Stereology Andrology Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Testis medicine Animals Spermatogenesis Busulfan Infertility Male Azoospermia 030102 biochemistry & molecular biology Chemistry Dimethyl sulfoxide Mesenchymal stem cell Mesenchymal Stem Cells General Medicine medicine.disease Sperm Medical Laboratory Technology Culture Media Conditioned 030220 oncology & carcinogenesis |
| Zdroj: | Biotechnic & Histochemistry. 97:107-117 |
| ISSN: | 1473-7760 1052-0295 |
| Popis: | Busulfan (BSU) is a chemotherapeutic drug that can cause subfertility or sterility in males. We investigated the effects of adipose tissue-derived mesenchymal stem cells (AT-MSC) conditioned medium (CM) (AT-MSC-CM) on histopathological and molecular characteristics of mouse testes exposed to BSU using stereology. We used adult male mice divided randomly into five groups: control, Dulbecco's modified Eagle's medium (DMEM), dimethyl sulfoxide (DMSO), BSU, and BSU + CM. Thirty-five days following BSU injection, sperm and testis tissues were harvested for stereological and molecular studies. The BSU group exhibited significantly reduced testis volume, interstitium and tubules compared to the other groups, although the volume of the testis remained unchanged for BSU and CM groups. The number of testis cells was reduced in the BSU group compared to the other groups. The CM group exhibited a significantly increased number of testis cells compared to the BSU group. Sperm count and motility, and length density of seminiferous tubules were increased in CM group compared to the BSU group. AT-MSC-CM exhibited ameliorative effects on histopathologic changes of mouse testes exposed to BSU. |
| Databáze: | OpenAIRE |
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