Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) influences adipocytes injuries through triggering XBP1 and activating mitochondria-mediated apoptosis
| Autor: | Jia-Yi Lu, Yingtao Lv, Pingping Xu, Jin-Jin Zeng, Xueyang Zhang, Yuan Su |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Histology XBP1 Lipopolysaccharide Physiology adipocytes Inflammation Diseases of the endocrine glands. Clinical endocrinology chemistry.chemical_compound pg-lps Internal medicine medicine QP1-981 Gene silencing Porphyromonas gingivalis QH573-671 Adiponectin biology Leptin apoptosis Cell Biology RC648-665 biology.organism_classification Endocrinology chemistry inflammation Apoptosis medicine.symptom Cytology xbp1 Research Article Research Paper |
| Zdroj: | Adipocyte, Vol 10, Iss 1, Pp 28-37 (2021) Adipocyte article-version (VoR) Version of Record |
| ISSN: | 2162-397X 2162-3945 |
| DOI: | 10.1080/21623945.2020.1856527 |
| Popis: | Obesity is an important public-health problem worldwide. This study aimed to determine effects of porphyromonas gingivalis lipopolysaccharide (Pg-LPS) on adipocytes injuries and explore associated mechanisms. Adipocytes were isolated from SD rats. pLVX-XBP1 (XBP1 over-expression) and pLVX-XBP1-RNAi (silencing XBP1) were structured and transfected into adipocytes. All adipocytes were divided into pLVX-NC, pLVX-XBP1, pLVX-NC+Pg-LPS and pLVX-XBP1+ Pg-LPS group. Oil-Red O staining was employed to identify isolated adipocytes. Quantitative real-time PCR (qRT-PCR) was used to examine gene transcription of IL-6, TNF-α, leptin, adiponectin. Western blotting was used to detect Bax and caspase-3 expression. Adipocytes were successfully isolated and identified with Oil-Red O staining. Both XBP1 mimic and XBP1 RNAi were effectively transfected into adipocytes with higher expressing efficacy. XBP1 over-expression significantly aggravated Pg-LPS induced inflammatory response compared to adipocytes without Pg-LPS treatment (p |
| Databáze: | OpenAIRE |
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