Structure of the ribozyme substrate hairpin of Neurospora VS RNA: A close look at the cleavage site
| Autor: | Caroline H. J. Schouten, Cornelis W. Hilbers, Hans A. Heus, Paul J. A. Michiels |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Models
Molecular Hammerhead ribozyme Magnetic Resonance Spectroscopy Stereochemistry Base pair Molecular Sequence Data Cleavage (embryo) Substrate Specificity Structure-Activity Relationship Magnesium RNA Catalytic Base Pairing Molecular Biology Binding Sites Base Sequence Neurospora crassa biology GIR1 branching ribozyme Hydrolysis Ribozyme RNA Fungal Stem-loop biology.organism_classification Biochemistry biology.protein Nucleic Acid Conformation RNA Satellite Hairpin ribozyme VS ribozyme Research Article |
| Zdroj: | RNA. 6:1821-1832 |
| ISSN: | 1355-8382 |
| DOI: | 10.1017/s1355838200001394 |
| Popis: | The cleavage site of the Neurospora VS RNA ribozyme is located in a separate hairpin domain containing a hexanucleotide internal loop with an A-C mismatch and two adjacent G-A mismatches. The solution structure of the internal loop and helix la of the ribozyme substrate hairpin has been determined by nuclear magnetic resonance (NMR) spectroscopy. The 2 nt in the internal loop, flanking the cleavage site, a guanine and adenine, are involved in two sheared G.A base pairs similar to the magnesium ion-binding site of the hammerhead ribozyme. Adjacent to the tandem G.A base pairs, the adenine and cytidine, which are important for cleavage, form a noncanonical wobble A+-C base pair. The dynamic properties of the internal loop and details of the high-resolution structure support the view that the hairpin structure represents a ground state, which has to undergo a conformational change prior to cleavage. Results of chemical modification and mutagenesis data of the Neurospora VS RNA ribozyme can be explained in context with the present three-dimensional structure. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|