Determination of naproxen using DBS: evaluation & pharmacokinetic comparison of human plasma versus human blood DBS
| Autor: | Nikolay Youhnovski, Marc Lefebvre, Pascal Guibord, Jean-Nicholas Mess, Robert B Macarthur, Annik Bergeron, Marianne Rufiange, Milton Furtado, Fabio Garofolo, Josée Michon, Sylvain Latour |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Bioanalysis Naproxen Clinical Biochemistry Pharmacology behavioral disciplines and activities Analytical Chemistry Drug Stability Pharmacokinetics Tandem Mass Spectrometry medicine Chemical Precipitation Humans Desiccation General Pharmacology Toxicology and Pharmaceutics Dried blood Blood Specimen Collection Chromatography Human blood Chemistry Reproducibility of Results Blood Proteins General Medicine Reference Standards Quantitative determination nervous system diseases Bioavailability Medical Laboratory Technology surgical procedures operative Solubility nervous system Human plasma Linear Models Regression Analysis Female Controlled Clinical Trials as Topic therapeutics Blood Chemical Analysis Chromatography Liquid medicine.drug |
| Zdroj: | Bioanalysis. 2:1501-1513 |
| ISSN: | 1757-6199 1757-6180 |
| Popis: | Background: Dried blood spots (DBS) sampling is a well-known technology for qualitative determination such as DNA analysis and screening of newborn metabolic disorders. The scientific community has recently expressed interest in applying the DBS technique for quantitative determination of drugs in biological fluid. Results: Two new bioanalytical assays were developed and validated for the determination of naproxen in human plasma and in DBS samples using liquid chromatography coupled with tandem MS. Furthermore, plasma and DBS clinical samples were collected from four subjects enrolled as part of a bioequivalence study. Concentration data for plasma and DBS samples were determined and pharmacokinetic (PK) profiles in plasma and in DBS samples were compared. Conclusions: A strong correlation between PK data obtained by the DBS and conventional plasma method was observed, which makes DBS a valuable technique for further naproxen bioavailability and PK investigations and studies. |
| Databáze: | OpenAIRE |
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