Tissue Microarrays to Visualize Influenza D Attachment to Host Receptors in the Respiratory Tract of Farm Animals
| Autor: | Nemanichvili, Nikoloz, Berends, Alinda J, Wubbolts, Richard W, Gröne, Andrea, Rijks, Jolianne M, de Vries, Robert P, Verheije, Monique H, VP pathologie, dI&I I&I-1, dPB I&I, Celbiologie, IOV CCB, dB&C I&I, dPB CR, VPDC pathologie, Afd Chemical Biology and Drug Discovery, Pharmacology, Chemical Biology and Drug Discovery |
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| Přispěvatelé: | VP pathologie, dI&I I&I-1, dPB I&I, Celbiologie, IOV CCB, dB&C I&I, dPB CR, VPDC pathologie, Afd Chemical Biology and Drug Discovery, Pharmacology, Chemical Biology and Drug Discovery |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Farm animals Hemagglutination Swine 030106 microbiology Respiratory System lcsh:QR1-502 Host tropism Hemagglutinins Viral Virus Attachment Biology Influenza D Virus lcsh:Microbiology Article Microbiology Tissue microarray 03 medical and health sciences 9-O-acetylated sialic acid Virology medicine Animals Horses Receptor Tropism Sheep Host Microbial Interactions Goats Epithelium Recombinant Proteins Viral Tropism 030104 developmental biology medicine.anatomical_structure Infectious Diseases Tissue Array Analysis Animals Domestic Tissue tropism biology.protein Sialic Acids Cattle Antibody Thogotovirus Viral Fusion Proteins |
| Zdroj: | Viruses Volume 13 Issue 4 Viruses, 13(4), 1. Multidisciplinary Digital Publishing Institute (MDPI) Viruses, Vol 13, Iss 586, p 586 (2021) |
| ISSN: | 1999-4915 |
| Popis: | The trimeric hemagglutinin-esterase fusion protein (HEF) of influenza D virus (IDV) binds 9-O-acetylated sialic acid receptors, which are expressed in various host species. While cattle are the main reservoir for IDV, the viral genome has also been detected in domestic pigs. In addition, antibodies against IDV have been detected in other farm animals such as sheep, goats, and horses, and even in farmers working with IDV positive animals. Viruses belonging to various IDV clades circulate, but little is known about their differences in host and tissue tropism. Here we used recombinantly produced HEF proteins (HEF S57A) from the major clades D/Oklahoma (D/OK) and D/Oklahoma/660 (D/660) to study their host and tissue tropism and receptor interactions. To this end, we developed tissue microarrays (TMA) composed of respiratory tissues from various farm animals including cattle, domestic pigs, sheep, goats, and horses. Protein histochemical staining of farm animal respiratory tissue-microarrays with HEF proteins showed that cattle have receptors present over the entire respiratory tract while receptors are only present in the nasal and pharyngeal epithelium of pigs, sheep, goats, and horses. No differences in tropism for tissues and animals were observed between clades, while hemagglutination assays showed that D/OK has a 2-fold higher binding affinity than D/660 for receptors on red blood cells. The removal of O-acetylation from receptors via saponification treatment confirmed that receptor-binding of both clades was dependent on O-acetylated sialic acids. |
| Databáze: | OpenAIRE |
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