Penicillin-binding protein 4 overproduction increases beta-lactam resistance in Staphylococcus aureus
| Autor: | Brigitte Berger-Bächi, U U Henze |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
DNA
Bacterial Staphylococcus aureus Penicillin binding proteins Transcription Genetic Molecular Sequence Data Mutant Microbial Sensitivity Tests Penicillins Muramoylpentapeptide Carboxypeptidase Biology medicine.disease_cause Microbiology Plasmid Bacterial Proteins Transcription (biology) medicine Penicillin-Binding Proteins Pharmacology (medical) Amino Acid Sequence Promoter Regions Genetic Gene Antibacterial agent Pharmacology Base Sequence Genetic Complementation Test Promoter Chromosomes Bacterial Molecular biology Infectious Diseases Hexosyltransferases Peptidyl Transferases ATP-Binding Cassette Transporters Methicillin Resistance Carrier Proteins Research Article Plasmids |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 40:2121-2125 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.40.9.2121 |
| Popis: | The Staphylococcus aureus mutant strain PVI selected in vitro for methicillin resistance overexpressed penicillin-binding protein (PBP) 4. In the wild-type parent strain the pbp4 gene was separated by 419 nucleotides from a divergently transcribed abcA locus coding for an ATP-binding cassette transporter. The mutant PVI was shown to have a deletion in the pbp4-abcA promoter region that affected pbp4 transcription but not expression of abcA. Introduction of the pbp4 gene plus the mutant promoter region into different genetic backgrounds revealed that PBP 4 overproduction was sufficient to increase in vitro-acquired methicillin resistance independently of other chromosomal genes. The role of the AbcA transporter in methicillin resistance remained unknown. |
| Databáze: | OpenAIRE |
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