An in vitro system for the study of slow axonal transport
| Autor: | Martin Kanje, Pär Remgård, W. G. McLean |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Nerve Tissue Proteins
In Vitro Techniques Sulfur Radioisotopes Axonal Transport Rats Sprague-Dawley Methionine Slow axonal transport medicine Animals Molecular Biology Polyacrylamide gel electrophoresis biology General Neuroscience Vagus Nerve Nodose Ganglion Axons In vitro Rats Blot Tubulin medicine.anatomical_structure Peripheral nervous system biology.protein Biophysics Axoplasmic transport Autoradiography Electrophoresis Polyacrylamide Gel Female Neurology (clinical) Neuroscience Developmental Biology |
| Zdroj: | Brain Research. 613:295-299 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/0006-8993(93)90914-9 |
| Popis: | Here we present a model system which for the first time permits studies of slow axonal transport in vitro. Axonally transported proteins of rat vagus nerves were radiolabelled with [35S]methionine in the nodose ganglion in vitro and were incubated for up to 3 days in culture medium. Slowly transported proteins were analyzed by one- and two-dimensional polyacrylamide gel electrophoresis and identified on Western blots of two-dimensional gels with antibodies to actin and alpha-tubulin. The system will be valuable for pharmacological analysis of the mechanisms of slow transport. |
| Databáze: | OpenAIRE |
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