Improved cellular uptake of perfluorocarbon nanoparticles for in vivo murine cardiac 19F MRS/MRI and temporal tracking of progenitor cells
| Autor: | Louiza Potamiti, Ayman Al Haj Zen, Andrew M. Shaw, Eileen McNeill, Christakis Constantinides, Raquel Sainz-Urruela, Sergi Padilla-Parra, Kyriacos Kyriacou, Mangala Srinivas, Jyoti Patel, Ricardo Carnicer, Andreas Hadjisavvas, Carolyn A. Carr, Rita Alonaizan, Edyta Swider |
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| Rok vydání: | 2019 |
| Předmět: |
Cancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2]
Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering 02 engineering and technology Flow cytometry law.invention 03 medical and health sciences All institutes and research themes of the Radboud University Medical Center Confocal microscopy law In vivo FuGENE Fluorescence microscope medicine General Materials Science Progenitor cell 030304 developmental biology 0303 health sciences medicine.diagnostic_test Chemistry Histology 021001 nanoscience & nanotechnology In vitro 3. Good health Molecular Medicine 0210 nano-technology Nanomedicine Radboud Institute for Molecular Life Sciences [Radboudumc 19] Biomedical engineering |
| Zdroj: | Nanomedicine: Nanotechnology, Biology and Medicine Nanomedicine-Nanotechnology Biology and Medicine, 18, pp. 391-401 Nanomedicine-Nanotechnology Biology and Medicine, 18, 391-401 |
| ISSN: | 1549-9634 |
| DOI: | 10.1016/j.nano.2018.10.014 |
| Popis: | Herein, we maximize the labeling efficiency of cardiac progenitor cells (CPCs) using perfluorocarbon nanoparticles (PFCE-NP) and 19F MRI detectability, determine the temporal dynamics of single-cell label uptake, quantify the temporal viability/fluorescence persistence of labeled CPCs in vitro, and implement in vivo, murine cardiac CPC MRI/tracking that could be translatable to humans. FuGENEHD-mediated CPC PFCE-NP uptake is confirmed with flow cytometry/confocal microscopy. Epifluorescence imaging assessed temporal viability/fluorescence (up to 7 days [D]). Nonlocalized murine 19F MRS and cardiac MRI studied label localization in terminal/longitudinal tracking studies at 9.4 T (D1-D8). A 4-8 fold 19F concentration increase is evidenced in CPCs for FuGENE vs. directly labeled cells. Cardiac 19F signals post-CPC injections diminished in vivo to ~31% of their values on D1 by D7/D8. Histology confirmed CPC retention, dispersion, and macrophage-induced infiltration. Intra-cardiac injections of PFCE-NP-labeled CPCs with FuGENE can be visualized/tracked in vivo for the first time with 19F MRI. |
| Databáze: | OpenAIRE |
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