Optimization of the Cuff Technique for Murine Heart Transplantation
| Autor: | Zhongquan Qi, Helong Dai, Tian-Shu Lan, Shuangyue Xu, Baiyi Xie, Guoliang Yan, Shujiao Liu, Yunhan Ma, Chenxi Wang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Microsurgery General Chemical Engineering medicine.medical_treatment General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Mice 0302 clinical medicine Basic research medicine Operation time Animals Heart transplantation General Immunology and Microbiology business.industry General Neuroscience Surgery Transplantation Disease Models Animal 030104 developmental biology medicine.anatomical_structure Cuff cardiovascular system Heart Transplantation business External jugular vein 030215 immunology Blood vessel |
| Zdroj: | Journal of visualized experiments : JoVE. (160) |
| ISSN: | 1940-087X |
| Popis: | Murine cardiac transplantation has been performed for more than 40 years. With advancements in microsurgery, certain new techniques have been used to improve surgical efficiency. In our lab, we have optimized the cuff technique with two major steps. First, we used the inner tube technique to insert a temporary inner tube into the external jugular vein and carotid artery blood vessel to facilitate eversion of the vessel over the cuff. Second, we performed complete heterotopic cardiac transplantation through the collaboration of two experienced surgeons. These modifications effectively reduced the operation time to 25 minutes, with a success rate of 95%. In this report, we describe these procedures in detail and provide a supplemental video. We believe that this report on the improved cuff technique will offer practical guidance for murine heterotopic heart transplantation and will enhance the utility of this mouse model for basic research. |
| Databáze: | OpenAIRE |
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