Characterization of M Gene-Deficient Rabies Virus with Advantages of Effective Immunization and Safety as a Vaccine Strain
| Autor: | Kenta Shimizu, Nobuyuki Minamoto, Naoto Ito, Kentaro Yamada, Junji Hosokawa, Mutsuyo Takayama-Ito, Makoto Sugiyama |
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| Rok vydání: | 2005 |
| Předmět: |
Genes
Viral Immunology Antibodies Viral medicine.disease_cause Microbiology Virus Cell Line Viral Matrix Proteins Mice Rabies vaccine Cell Line Tumor Cricetinae Virology medicine Baby hamster kidney cell Animals Neutralizing antibody Administration Intranasal Duck embryo vaccine Mice Inbred BALB C biology Rabies virus Antibody titer Rabies Vaccines Immunization biology.protein medicine.drug |
| Zdroj: | Microbiology and Immunology. 49:971-979 |
| ISSN: | 0385-5600 |
| Popis: | Matrix (M) protein of rabies virus is known to play an important role in assembly and budding of the progeny virus. We generated an M gene-deficient rabies virus, RC-HLDeltaM, using a reverse genetics system of rabies virus RC-HL strain to develop a novel type of vaccine. RC-HLDeltaM infection was confined within a single cell in mouse neuroblastoma cells. This deficient virus failed to generate the progeny virus in the cells. In contrast, RC-HLDeltaM propagated in BHK cells inductively expressing M protein. Suckling and adult mice inoculated intracerebrally with the parental RC-HL strain showed lethal infection and transient body weight loss, respectively, whereas both suckling and adult mice inoculated with RC-HLDeltaM showed no symptoms. The neutralizing antibody against rabies virus was successfully induced by intramuscular immunization with 10(5) focus-forming units of RC-HLDeltaM but not UV-inactivated RC-HL. Intranasal immunization with RC-HLDeltaM resulted in almost the same antibody titer to rabies virus as that in the case of immunization with live RC-HL strain. These findings indicate that RC-HLDeltaM is a candidate for a novel rabies vaccine that is safer and more effective than are current vaccines. |
| Databáze: | OpenAIRE |
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