Vaccination in Humans Generates Broad T Cell Cytokine Responses
| Autor: | Mario Roederer, Fabien X. Lü, Richard A. Koup, Stephen P. Perfetto, Susan Moser, Joanne Yu, Judith Falloon, Christopher J. Miller, Thomas G. Evans, Stephen C. De Rosa |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
CD4-Positive T-Lymphocytes
Chemokine Subdominant medicine.medical_treatment T cell Immunology Immunization Secondary Epitopes T-Lymphocyte HIV Infections Lymphocyte Activation T-Lymphocytes Regulatory Epitope Immunophenotyping Interferon-gamma Immune system T-Lymphocyte Subsets Tetanus Toxoid medicine Humans Immunology and Allergy Hepatitis B Vaccines AIDS Vaccines biology T-Lymphocytes Helper-Inducer Flow Cytometry Vaccination Cytokine medicine.anatomical_structure Chronic Disease biology.protein Cytokines Interleukin-2 Immunologic Memory |
| Zdroj: | The Journal of Immunology. 173:5372-5380 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.173.9.5372 |
| Popis: | In recent years, the quantification of T cell responses to pathogens or immunogens has become a common tool in the evaluation of disease pathogenesis or vaccine immunogenicity. Such measurements are usually limited to enumerating IFN-γ-producing cells after ex vivo stimulation with Ag, but little is known about the phenotype or complete functional repertoire of the Ag-specific cells. We used 12-color flow cytometry to characterize Ag-specific T cells elicited by vaccines or natural infection to determine lineage and differentiation status as well as the capacity to produce four cytokines (IFN-γ, TNF-α, IL-2, and IL-4) and a chemokine (MIP1β). As expected, responding cells had a typical memory phenotype; however, the cytokine profiles associated with the responses were highly complex. The pattern of cytokine coexpression in response to specific Ags was a skewed subset of the complete repertoire (revealed by polyclonal stimulation). We found significant differences in the patterns of cytokines elicited by vaccination (where IFN-γ was by far a subdominant response) vs natural infection; in addition, there was fairly significant intersubject variation. Our findings illustrate the limitation of the evaluation of immune responses using single functional measurements (such as IFN-γ); in fact, it is likely that sensitive evaluation of Ag-specific T cells will require the coordinate measurement of several cytokines. The presence and variability of these complex response profiles introduce the possibility that selective functional expression patterns may provide correlates for vaccine efficacy or disease progression. |
| Databáze: | OpenAIRE |
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