Inhibition of osteoclast activities by SCPC bioceramic promotes osteoblast‐mediated graft resorption and osteogenic differentiation
| Autor: | Uruj Sarwar, Leire Bergara Muguruza, Mohammad Hassan, Randa Al Fotawi, Robert A. Horowitz, Miho Nakamura, Ahmed El-Ghannam |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Calcium Phosphates
Ceramics Materials science 0206 medical engineering Biomedical Engineering Osteoclasts Biocompatible Materials 02 engineering and technology Tartrate Mineralization (biology) Biomaterials chemistry.chemical_compound Osteogenesis Osteoclast medicine Humans Bone Resorption Cells Cultured Cell Nucleus Osteoblasts biology Spectrophotometry Atomic Metals and Alloys Bone Marrow Stem Cell Cell Differentiation Osteoblast Prostheses and Implants Silicon Dioxide 021001 nanoscience & nanotechnology Phosphate 020601 biomedical engineering Molecular biology Resorption medicine.anatomical_structure Gene Expression Regulation chemistry Ceramics and Composites Osteocalcin biology.protein Calcium 0210 nano-technology |
| Zdroj: | Journal of Biomedical Materials Research Part A. 109:1714-1725 |
| ISSN: | 1552-4965 1549-3296 |
| Popis: | Maximizing vital bone in a grafted site is dependent on a number of factors. These include resorption or turnover of the graft material, stimulation of bone formation pathway without a need for biological molecules added to the site and inhibition of cellular activities that compromise the mineralization of new bone matrix. In the present study, the dissolution profile of silica-calcium phosphate composite (SCPC) in physiological solution was measured and the data were fed to (ANN-NARX) prediction model to predict the time required for complete dissolution. The inductively coupled plasma-optical emission spectrometer ionic composition analysis of the culture medium incubated for 3 days with SCPC showed 57% decrease in Ca concentration and a significant increase in the concentration of Si (13.5 ± 1.8 μg/ml), P (249.4 ± 22 μg/ml), and Na (9.3 ± 0.52 μg/ml). In conjunction with the release of Si, P, and Na ions, the bone resorptive activity of osteoclasts was inhibited as indicated by the significant decrease in multinucleated tartrate resistant acidic phosphate stained cells and the volume of resorption pits on bone slices. In contrast, addition of SCPC to hBMSC cultured in conventional medium promoted higher Runt-related transcription factor 2 (p < .05), osteocalcin (p < .01), and bone sialo protein (p < .01) than that expressed by control cells grown in the absence of SCPC. The predicted dissolution time of 200 mg of porous SCPC particles in 10 ml phosphate buffered saline is 6.9 months. An important byproduct of the dissolution is inhibition of osteoclastic activity and promotion of osteoblastic differentiation and hence bone formation. |
| Databáze: | OpenAIRE |
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