DNA cleavage studies of mononuclear and dinuclear copper(II) complexes with benzothiazolesulfonamide ligands
Autor: | Bernard Meunier, Marguerite Pitié, Marta González-Álvarez, Gloria Alzuet, Joaquín Borrás |
---|---|
Rok vydání: | 2003 |
Předmět: |
Models
Molecular inorganic chemicals Stereochemistry Reducing agent Guanine Radical chemistry.chemical_element Ligands Cleavage (embryo) Photochemistry Biochemistry Inorganic Chemistry chemistry.chemical_compound Organometallic Compounds Reactivity (chemistry) Chromatography High Pressure Liquid Bond cleavage Sulfonamides Deoxyribonucleases Deoxyribose DNA Free Radical Scavengers Copper Thiazoles chemistry Reducing Agents Electrophoresis Polyacrylamide Gel Reactive Oxygen Species Oxidation-Reduction |
Zdroj: | JBIC Journal of Biological Inorganic Chemistry. 8:644-652 |
ISSN: | 1432-1327 0949-8257 |
Popis: | Copper-based transition metal complexes performing single- and double-strand scission of DNA have been studied. The dinuclear complexes [Cu(2)(L)(2)(OCH(3))(2)(NH(3))(2)] and [Cu(2)(L)(2)(OCH(3))(2)(DMSO)(2)] are more active than the corresponding mononuclear [Cu(L)(2)(py)(2)] (where HL= N-(4-methylbenzothiazol-2-yl)benzenesulfonamide), suggesting that the dinuclearity is an important factor in the oxidative cleavage of DNA. The cleavage efficiency of the complexes depends on the reducing agent used in the process, the tandem ascorbate/H(2)O(2) being the most efficient. PAGE analyses have shown that these complexes cleave DNA without sequence selectivity. The DNA degradation process takes place mainly by C1' oxidation, but C4' and C5' oxidations cannot be ruled out as minor pathways. These copper complexes preferably oxidize guanine under mild conditions, but under more drastic conditions the oxidation reactivity appears to be TGCA, suggesting the intervention of hydroxyl radicals as active species. |
Databáze: | OpenAIRE |
Externí odkaz: |