Cryopreservation and in vitro culture of white-tailed deer ovarian tissue
| Autor: | Jean M. Feugang, G. A. Apgar, Eduardo L. Gastal, W. J. Banz, G. D. A. Gastal, A.P.R. Rodrigues, F.L.N. Aguiar, J.M. Scimeca |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Biology
Cryopreservation Protein expression Tissue Culture Techniques Andrology 03 medical and health sciences 0302 clinical medicine Food Animals Animals Vitrification Small Animals 030219 obstetrics & reproductive medicine Equine Deer Ovarian tissue Ovary 0402 animal and dairy science 04 agricultural and veterinary sciences 040201 dairy & animal science In vitro White (mutation) Apoptosis Female Animal Science and Zoology Tissue Preservation |
| Zdroj: | Theriogenology. 113:253-260 |
| ISSN: | 0093-691X |
| DOI: | 10.1016/j.theriogenology.2018.03.003 |
| Popis: | The aims of this study were to evaluate (1) the survivability of white-tailed deer ovarian tissue after cryopreservation by slow-freezing (SF) and vitrification (VIT) techniques and in vitro culture (IVC) for up to 7 days, and (2) the effects of cryopreservation techniques on protein expression of proliferative and apoptotic markers of ovarian tissue pre- and post-in vitro culture. Ovaries (n = 14) of seven white-tailed deer fawns (1.5 years old) were used. Ovarian cortexes were cut into fragments (2 × 2 × 0.5 mm) and split into nine treatment groups: (1) fresh noncultured control, (2) fresh-IVC 1 day, (3) fresh-IVC 7 days, (4) SF noncultured, (5) SF-IVC 1 day, (6) SF-IVC 7 days, (7) VIT noncultured, (8) VIT-IVC 1 day, and (9) VIT-IVC 7 days. Preantral follicle morphology, class distribution, and density; stromal cell density; EGFR, Ki-67, Bax, and Bcl-2 protein expression; and DNA fragmentation were assessed. Results showed that: (i) white-tailed deer fresh ovarian tissue can be cultured for up to 7 days, preserving the tissue integrity and 50% of morphologically normal preantral follicles; (ii) cryopreservation of white-tailed deer ovarian tissue by either slow-freezing or vitrification does not disrupt markers of proliferation and apoptosis after thawing; (iii) ovarian fragments cryopreserved by the vitrification method had greater follicle viability during in vitro culture than the slow-freezing method; and (iv) fragments cryopreserved by slow-freezing suffered apoptosis earlier than those preserved by vitrification. The findings herein reported advance knowledge towards development of adequate cryopreservation protocols for long-term banking programs for Cervidae species. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect